| 不同剂量胰岛素、胰高血糖素和甲状腺素处理对奶牛乳腺上皮细胞FcRn mRNA表达的影响 |
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| 引用本文： | 胡小丽,张春林,王加启,赵圣国,章玉涛,卜登攀,周凌云.不同剂量胰岛素、胰高血糖素和甲状腺素处理对奶牛乳腺上皮细胞FcRn mRNA表达的影响[J].中国农业科学,2011,44(20):4279-4283. |
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| 作者姓名： | 胡小丽 张春林 王加启 赵圣国 章玉涛 卜登攀 周凌云 |
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| 作者单位： | 1.中国农业科学院北京畜牧兽医研究所/动物营养学国家重点实验室 |
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| 摘 要： | 【目的】采用real-time PCR方法检测体外激素处理对奶牛乳腺上皮细胞FcRn（neonatal Fc receptor，FcRn）mRNA表达的影响。【方法】在乳腺上皮细胞培养液中添加不同浓度胰高血糖素（0.01、0.1和1 μmol?L-1）、胰岛素（0.005、0.1和0.5 μmol?L-1）、甲状腺素T4（0.01、0.1和1 μmol?L-1），体外培养并收集细胞，提取细胞mRNA后，利用real-time PCR检测FcRn mRNA的相对丰度。【结果】在一定添加剂量范围内，随着添加剂量的增加，甲状腺素和胰高血糖素能够显著地促进FcRn mRNA表达量升高（P＜0.05），胰岛素在添加量为0.005 μmol?L-1和0.5 μmol?L-1时FcRn mRNA的表达量低，但在0.1 μmol?L-1添加时表达量显著升高（P＜0.05）。【结论】添加外源胰岛素、胰高血糖素和甲状腺素能够影响FcRn mRNA的表达，为进一步研究乳腺内FcRn受体变化以及影响因素提供了依据。
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| 关 键 词： | 荷斯坦奶牛 乳腺上皮细胞 激素 real-timePCR mRNA |
| 收稿时间： | 2010-08-30 |
The Expression of FcRn mRNA in Mammary Gland Epithelial Cells of Holstein Cows Treated with Different Concentrations of Thyroxine, Insulin, and Glucagon in Vitro |
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| HU Xiao-li,ZHANG Chun-lin,WANG Jia-qi,ZHAO Sheng-guo,ZHANG Yu-tao,BU Deng-pan,ZHOU Ling-yun.The Expression of FcRn mRNA in Mammary Gland Epithelial Cells of Holstein Cows Treated with Different Concentrations of Thyroxine, Insulin, and Glucagon in Vitro[J].Scientia Agricultura Sinica,2011,44(20):4279-4283. |
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| Authors: | HU Xiao-li ZHANG Chun-lin WANG Jia-qi ZHAO Sheng-guo ZHANG Yu-tao BU Deng-pan ZHOU Ling-yun |
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| Institution: | HU Xiao-li,ZHANG Chun-lin,WANG Jia-qi,ZHAO Sheng-guo,ZHANG Yu-tao,BU Deng-pan,ZHOU Ling-yun(Institute of Animal Science,Chinese Academy of Agriculture Science/State Key Lab of Animal Nutrition,Beijing 100193) |
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| Abstract: | 【Objective】The mRNA expression of FcRn was studied in Holstein Cows mammary gland epithelial cells treated with hormone by real-time PCR. 【Method】 FcRn mRNA expression of in vitro cultured mammary epithelial cells treated with different concentrations of thyroxine hormone (0.01, 0.1, 1 μmol?L-1), insulin (0.005, 0.1, 0.5 μmol?L-1) and glucagon (0.01, 0.1, 1 μmol?L-1), respectively, was determined.【Result】The results showed that the mRNA abundance of FcRn increased with the higher dose of thyroxine and glucagon significantly (P＜0.05) in a certain range. However, the mRNA expression of FcRn was significantly lower when treated with 0.005 μmol?L-1 and 0.5 μmol?L-1 insulin than 0.1μmol?L-1 (P＜0.05).【Conclusion】Thyroxine, insulin and glucagon can obviously change the level of FcRn mRNA, which provide a basis for further research of the change and influence of FcRn. |
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| Keywords: | Holstein Cows mammary epithelial cells hormone real-time PCR mRNA |
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