| 重组N蛋白抗原检测PRRSV抗体间接ELISA方法的建立 |
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| 引用本文： | 李素平,赵琳,夏平安,汪银锋,王中明,邱璜,刘明莉,徐红运.重组N蛋白抗原检测PRRSV抗体间接ELISA方法的建立[J].江西农业学报,2009,21(3). |
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| 作者姓名： | 李素平 赵琳 夏平安 汪银锋 王中明 邱璜 刘明莉 徐红运 |
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| 作者单位： | 河南农业大学,牧医工程学院,河南,郑州,450002 |
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| 摘 要： | 采用纯化猪繁殖与呼吸综合征病毒重组核衣壳蛋白作为ELISA试验的标准抗原,通过优化ELISA各种条件,建立了检测PRRSV抗体的间接ELISA方法。该方法具有良好的特异性和重复性。
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| 关 键 词： | 猪繁殖与呼吸综合征病毒 N蛋白 间接ELISA |
Establishment of Indirect ELISA Method for Porcine Reproductive and Respiratory Syndrome Virus Recombinant Protein N |
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| LI Su-ping,ZHAO Lin,XIA Ping-an,WANG Yin-feng,WANG Zhong-ming,QIU Huang,LIU Ming-li,XU Hong-yun.Establishment of Indirect ELISA Method for Porcine Reproductive and Respiratory Syndrome Virus Recombinant Protein N[J].Acta Agriculturae Jiangxi,2009,21(3). |
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| Authors: | LI Su-ping ZHAO Lin XIA Ping-an WANG Yin-feng WANG Zhong-ming QIU Huang LIU Ming-li XU Hong-yun |
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| Institution: | College of Animal Husbandry and Veterinary Engineering;Henan Agricultural University;Zhengzhou 450002;China |
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| Abstract: | The test used the purified porcine reproductive and respiratory syndrome virus(PRRSV) recombinant nucleus capsid protein as the standard antigen of ELISA test,through optimizing all conditions of ELISA test,the indirect ELISA method for testing the antibody of PRSSV was established,this method had good specificity and reproducibility. |
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| Keywords: | PRRSV N-protein Indirect ELISA |
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