| 马铃薯叶片原生质体的游离纯化 |
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| 引用本文： | 邸宏,卢翠华,陈伊里.马铃薯叶片原生质体的游离纯化[J].东北农业大学学报,2008,39(6). |
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| 作者姓名： | 邸宏 卢翠华 陈伊里 |
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| 作者单位： | 东北农业大学农学院,哈尔滨,150030 |
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| 基金项目： | 黑龙江省教育厅科学技术研究项目 |
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| 摘 要： | 文章以马铃薯四倍体普通栽培品种Desiree、东农303和二倍体野生种S.pinnatisectum品系脱毒试管苗叶片为材料,研究了不同的酶组合、酶液浓度、酶解时间以及温度等因子对原生质体游离纯化的影响。结果表明,低温预处理有助于提高原生质体的产量;最佳酶液组合为纤维素酶1.0%+离析酶0.5%;对于四倍体栽培种较短的酶解时间(13 h)较为适宜,而野生种需要较长的酶解时间(18 h)。
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| 关 键 词： | 马铃薯 原生质体 游离 |
Isolation and purification of protoplasts from potato leaf |
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| DI Hong,LU Cuihua,CHEN Yili.Isolation and purification of protoplasts from potato leaf[J].Journal of Northeast Agricultural University,2008,39(6). |
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| Authors: | DI Hong LU Cuihua CHEN Yili |
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| Abstract: | Using potato cultivar Desiree,Dongnong303 and plantlet's leaf,the various influencing factors on protoplast isolation and purification,such as the concentration and combination of enzyme,dissociation time,and dissociation temperature,were studied.The results showed that the low temperature pretreatment could increase the yield of protoplast.The optimum concentration and combination of enzyme were cellulase R-10 1.0 % macerozyme R-10 0.5%.The suitable dissociation time was 13 h for potato tetraploid cultivars,and 18 h for the diploid wild species. |
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| Keywords: | potato protoplast isolation |
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