| 羽衣甘蓝Exo70A1基因的分离及表达分析 |
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| 引用本文： | 杨佳,蓝兴国,郝艾馨,李玉花.羽衣甘蓝Exo70A1基因的分离及表达分析[J].园艺学报,2012,39(1):127-135. |
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| 作者姓名： | 杨佳 蓝兴国 郝艾馨 李玉花 |
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| 作者单位： | （东北林业大学生命科学学院，哈尔滨 150040） |
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| 基金项目： | 国家自然科学基金项目，中央高校基本科研业务费专项基金项目 |
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| 摘 要： | 以羽衣甘蓝（Brassica oleracea var. acephala）自交不亲和系（S13-b S13-b）为试材，从柱头中分离Exo70A1基因，命名为BoExo70A1，GenBank登录号为JF919716。BoExo70A1全长cDNA序列为2 184 bp，包含56 bp的5′非编码区，211 bp的3′非编码区和一个长度为1 917 bp的开放读码框（ORF），对应编码一个含有638个氨基酸残基的蛋白质。氨基酸序列比对分析表明，羽衣甘蓝BoExo70A1与油菜BnExo70A1、拟南芥AtExo70A1的一致性分别为99%和94%；BoExo70A1基因结构含12个外显子和11个内含子，内含子5′供体位点和3′受体位点边界序列符合GU-AG规则；Southern杂交结果显示，BoExo70A1在羽衣甘蓝基因组中存在多拷贝；Northern杂交结果显示BoExo70A1在茎、叶、花瓣、花药、柱头、花柱和子房中表达，而且在叶中的表达量最低。
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| 关 键 词： | 羽衣甘蓝 Exo70A1 序列分析 表达分析 |
| 收稿时间： | 2011-8-1 |
| 修稿时间： | 2011-11-30 |
Isolation and Expression Analysis of Exo7OA1 of Ornamental Kale |
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| YANG Jia,LAN Xing-guo ,HAOAi-xin,and LI Yu-hua.Isolation and Expression Analysis of Exo7OA1 of Ornamental Kale[J].Acta Horticulturae Sinica,2012,39(1):127-135. |
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| Authors: | YANG Jia LAN Xing-guo HAOAi-xin and LI Yu-hua |
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| Institution: | （College of Life Sciences，Northeast Forestry University，Harbin 150040，China） |
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| Abstract: | The Exo70A1 gene(BoExo70A1)was isolated from stigma of ornamental kale(Brassica oleracea var.acephala)S13-b homozygotes,whose GenBank accession number is JF919716.BoExo70A1 cDNA was 2 184 bp in the full length,which contained a 5′-untranslated region(5′-UTR)of 56 bp,a 3′-UTR of 211 bp,and an opening reading frame of 1 917 bp encoding a 638 predicted amino acids residue protein.The deduced amino acid sequence of BoExo70A1 shared 99%and 94%identity with Brassica napus Exo70A1 and Arabidopsis thaliana Exo70A1,respectively.The genomic structure of BoExo70A1 contained 12 exons and 11 introns with boundary sequences of GU at 5′site and AG at 3′site,which complied with the typical‘GU-AG rule’in plants.Southern hybridization analysis showed that there were multiple copies of BoExo70A1 gene in genome.Northern hybridization analysis showed that the BoExo70A1 was ubiquitous expressed in stem,petal,anther,stigma,style and ovary,as well as a relative low expression in leaf. |
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| Keywords: | Brassica oleracea var acephala Exo 70A 1 sequence analysis expression analysis |
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