Cloning and characterization of cDNA for carp matrix metalloproteinase 9 |
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| Authors: | Kazuharu TAKEUCHI Satoshi KUBOTA Masato KINOSHITA Haruhiko TOYOHARA AND Morihiko SAKAGUCHI |
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| Institution: | Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Kyoto, Kyoto 606-8502 and; Laboratory of Aquatic Product Utilization, Faculty of Agriculture, Kochi University, Nankoku, Kochi 783-8502, Japan |
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| Abstract: | ABSTRACT: We have cloned a cDNA encoding the MMP-9 from a carp epidermal cell (EPC) cDNA library. The clone contains a 2025-base pair (bp) open reading frame encoding a protein of 674 amino acids. The deduced amino acid sequence shares 68% and 69% identity with medaka and Japanese flounder MMP-9. The hinge domain of the carp MMP-9, like those of the other non-mammalian species, lacks a type V collagen-like region that is typical of mammalian MMP-9. Gelatin zymography and immunoblot analysis of conditioned media of EPC cells and cDNA-transfected COS-7 cells detected a 76-kDa gelatinase. The apparent molecular mass of the carp zymogen is much smaller than those of its mammalian counterparts while almost identical with that of chicken 75-kDa gelatinase B-like enzyme. Although hypo-osmotic stress induced the elevation of MMP-9 mRNA level in EPC cells, no significant change in the protein in conditioned medium was detected during hypo-osmotic stress. Northern blot analysis detected a large amount of MMP-9 mRNA in carp kidney and spleen, suggesting the high expression of MMP-9 in blood cells, neutrophils, and macrophages. The smaller amount of MMP-9 mRNA was detected in gill, heart, fin, and eye, whereas none of the mRNA was detected in the hepatopancreas, intestine, brain, muscle, and skin. |
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| Keywords: | common carp fish cells gelatinase matrix metalloproteinase |
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