| 16种四川道地药材对鸡胚成纤维细胞生长的影响 |
| |
| 引用本文： | 封海波,刘 娟,曾宪垠,赵 冰,唐 静,王振华,杜林林,吕 雪.16种四川道地药材对鸡胚成纤维细胞生长的影响[J].西南农业大学学报,2012,34(6):006-011. |
| |
| 作者姓名： | 封海波 刘 娟 曾宪垠 赵 冰 唐 静 王振华 杜林林 吕 雪 |
| |
| 作者单位： | 四川农业大学动物医学院;西南大学(荣昌校区)动物医学系 |
| |
| 基金项目： | 国家自然科学基金面上项目(31172357);西南大学青年基金资助项目(QNRF200906) |
| |
| 摘 要： | 该实验通过MTT法观察丹参、川木香、菊花、川郁金、川黄柏、川佛手、川牛膝、川莪术、川明参、川续断、川麦冬、川白芷、川芎、杜仲、川党参和乌梅等16种四川产道地药材对体外培养的鸡胚成纤维细胞(CEF)的安全体积质量分数,并观察各种中药提取液对CEF增殖的影响.结果表明以上各种药材的最大安全体积质量分数:川黄柏为3.907mg/L,丹参、川木香、川续断和川党参均为7.813mg/L,菊花为15.625mg/L,杜仲、川佛手、川麦冬、川芎和白芷均为31.25mg/L,川郁金、杜仲、川乌梅和川牛膝均为62.5mg/L,川明参为1 000mg/L.当川郁金在1.954～31.25mg/L,川牛膝在3.907～31.25mg/L,川明参在1.954～500mg/L,川续断在1.954～3.907mg/L,川麦冬在1.954～15.625mg/L,杜仲在1.954～31.25mg/L的体积质量分数范围内可显著促进细胞生长.
|
| 关 键 词： | 四川道地药材 鸡胚成纤维细胞 细胞增殖 安全体积质量分数 |
EffectsofSixteenSichuanHerbalMedicine
ExtractsonProliferationofChickEmbryoFibroblasts |
| |
| Institution: | FENG Hai-bo 1,2,LIU Juan 2,ZENG Xian-yin 1,ZHAO Bing 1,TANG Jing 1,WANG Zhen-hua 1,DU Lin-lin 2,L Xue 2 1.College of Veterinary Medicine,Sichuan Agricultural University,Yaan Sichuan 625014,China;2.Department of Veterinary Medicine,Southwest University(Rongchang Campus),Chongqing 402460,China |
| |
| Abstract: | An experiment was conducted to study the maximal safety concentrations of 16Sichuan herba1 medicines(danshen root,common Dolomiaea souliei root,chrysanthemum,Turmeric root-tuber,Amur corktree bark,finger citron,medicinal Cyathularoot,zedoary root,Himalayan teasel root,dwarf lilyturf tuber,Dahurian angelicaroot,Szechuan lovage,rhizome,Eucommia bark,Pilose asiabell root and dark plum fruit) for in vitro chick embryo fibroblasts(CEF) by MTT assay,and to evaluate the effects of these herba1 medicines on CEF proliferation.The results showed that the maximal safety concentration was 3.907mg/L for Amur corktree bark,7.813mg/L for danshen root,common Dolomiaea souliei root,Himalayan teasel root and Pilose asiabell root,15.625mg/L for chrysanthemum,31.25mg/L for finger citron,dwarf lilyturf tuber and Szechuan lovage rhizome,62.5mg/L for Eucommia bark,dark plum fruit and medicinal Cyathula root,and 1000mg/L for medicinal Changium root.Turmeric root-tuber at 1.954～31.25mg/L,medicinal Changium root at 1.954～500mg/L,cochin Chinese asparagus root at 1.954～15.625mg/L,Himalayan teasel root at 1.954～3.907mg/L,Medicinal Cyathula root at 3.907～31.25mg/L,dwarf lilyturf tuber at 1.954～15.625mg/L and Eucommia bark at 1.954～31.25mg/L promoted the cell growth significantly. |
| |
| Keywords: | Sichuan herbal medicine chick embryo fibroblast cell proliferation safety concentration |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《西南农业大学学报》浏览原始摘要信息 |
| 点击此处可从《西南农业大学学报》下载免费的PDF全文 |
|
