| ITGB1基因表达与鹅肥肝形成的关系 |
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| 引用本文： | 顾旺,张宜辉,张军,耿拓宇,龚道清.ITGB1基因表达与鹅肥肝形成的关系[J].安徽农业大学学报,2021,48(5):771-776. |
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| 作者姓名： | 顾旺 张宜辉 张军 耿拓宇 龚道清 |
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| 作者单位： | 扬州大学动物科学与技术学院,扬州225009 |
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| 基金项目： | 国家自然科学基金项目（31672405, 31972546）资助。 |
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| 摘 要： | 为探究ITGB1基因与鹅肥肝形成的关系,选取30只70日龄健康朗德公鹅,随机分为填饲组与对照组并进行24 d填饲.运用ITGB1基因序列进行生物信息学分析,采用RT-qPCR技术检测不同填饲阶段(填饲12d与24d)鹅肝脏中ITGB1基因的表达水平,同时,使用不同剂量的脂肪肝形成相关因子(胰岛素、葡萄糖、油酸、亚油酸和棕榈酸)处理鹅原代肝细胞,检测ITGB1基因的表达水平.结果 发现:全长CDS共2418 bp,编码805个氨基酸,与鸭的同源性高于98％,可编码1种亲水性蛋白;与对照相比,填饲12d与24 d时,鹅肝脏中ITGB1基因的表达均显著上调(P＜0.05);0.5 mmol·L-1油酸、亚油酸和棕榈酸均能显著地诱导鹅原代肝细胞中ITGB1基因上调(P＜0.05);200mmol·L-1葡萄糖能极显著地诱导鹅原代肝细胞中ITGB1基因上调(P＜0.01);此外,0.2 mmol·L-1胰岛素也能在一定程度上有诱导其表达上调的趋势(P=0.098).结果 表明,ITGB1基因表达上调与鹅肥肝形成密切相关,受脂肪肝形成相关因子诱导,提示ITGB1基因表达上调可能会促进鹅肥肝的形成.
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| 关 键 词： | 鹅 ITGB1 肥肝 基因表达 |
| 收稿时间： | 2020/6/13 0:00:00 |
The relationship between ITGB1 gene expression and the formation of goose fatty liver |
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| GU Wang,ZHANG Yihui,ZHANG Jun,GENG Tuoyu,GONG Daoqing.The relationship between ITGB1 gene expression and the formation of goose fatty liver[J].Journal of Anhui Agricultural University,2021,48(5):771-776. |
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| Authors: | GU Wang ZHANG Yihui ZHANG Jun GENG Tuoyu GONG Daoqing |
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| Institution: | College of Animal Science and Technology, Yangzhou University, Yangzhou 225009 |
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| Abstract: | To explore the relationship between ITGB1 gene and goose fatty liver formation, thirty healthy 70-day-old male Landes geese were randomly divided into a control group and an overfed group for 24 days of overfeeding. The sequence of ITGB1 gene was used for bioinformatics analysis, and its expression levels in the liver of geese at different overfeeding stages (12 and 24 days) were measured by RT-qPCR. Meanwhile, different doses of fatty liver formation related factors (insulin, glucose, oleic acid, linoleic acid, and palmitic acid) were used to treat goose primary hepatocytes to detect the expression levels of ITGB1 gene. The results showed that the total length of CDS is 2 418 bp, encoding 805 amino acids (which is a hydrophilic protein) and the homology between goose and duck is higher than 98 %. The expression of ITGB1 gene in goose fatty liver was significantly up-regulated (P<0.05) after 12 and 24 days of overfeeding compared with the control group. And 0.5 mmol·L-1 oleic acid, linoleic acid and palmitic acid could significantly induce the up-regulated expression of ITGB1 gene in goose primary hepatocytes (P< 0.05). Moreover, 200 mmol·L-1 glucose could also extremely significantly induce the up-regulated expression of ITGB1 gene in goose primary hepatocytes (P< 0.01). Beside, 0.2 mmol·L-1 insulin could also somehow have tend to induce the up-regulated expression of ITGB1 gene (P= 0.098). The results indicated that the up-regulation of ITGB1 gene was closely related to the formation of goose fatty liver and could be induced by factors related to fatty liver formation, which suggested that up-regulation of ITGB1 gene expression may promote the formation of goose fatty liver. |
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| Keywords: | goose ITGB1 fatty liver gene expression |
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