| 关木通中马兜铃酸A的提取及含量测定 |
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| 引用本文： | 王典仁,王彦英,陆彦,沈红,张中文,吴国娟.关木通中马兜铃酸A的提取及含量测定[J].中国畜牧兽医,2008,35(4):147-149. |
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| 作者姓名： | 王典仁 王彦英 陆彦 沈红 张中文 吴国娟 |
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| 作者单位： | 北京农学院动物科学技术系药理实验室，北京 102206 |
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| 基金项目： | 国家自然基金资助项目(30471306)，北京市自然基金项目(6053025) |
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| 摘 要： | 醇提中药关木通粉末，经旋转蒸发、真空干燥后得到粗提药物浸膏；在饱和NaHCO3溶液中溶解浸膏，静置分层后调节pH至3得到马兜铃总酸粗品；经硅胶G柱层析，洗脱液为氯仿∶乙酸乙酯∶苯（2∶1∶1），重结晶洗脱液得到黄色结晶体。反相高效液相色谱鉴定并测定结晶体中马兜铃酸A含量。结果显示黄色结晶体为马兜铃酸A单体，结晶体中马兜铃酸A的含量达99.2%，表明该提取方法可成功获得纯度较高的马兜铃酸A。
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| 关 键 词： | 关木通 马兜铃酸A 提取 含量测定 |
| 文章编号： | 1671-7236(2008)04-0147-03 |
| 修稿时间： | 2007年10月22 |
The Aristolochic Acid A'S Abstraction in Manchurian Dutchmanspipe Stem and Contet Assaying |
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| WANG Dian-ren,WANG Yan-ying,LU Yan,SHEN Hong,ZHANG Zhong-wen,WU Guo-juan.The Aristolochic Acid A'S Abstraction in Manchurian Dutchmanspipe Stem and Contet Assaying[J].China Animal Husbandry & Veterinary Medicine,2008,35(4):147-149. |
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| Authors: | WANG Dian-ren WANG Yan-ying LU Yan SHEN Hong ZHANG Zhong-wen WU Guo-juan |
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| Institution: | Beijing University of Agricultural Animal Science and Technical Department, Beijing 102206, China |
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| Abstract: | Extracted aristolochic con-acid by dehydrate alcohol as the solvent from 150 g manchurian dutchmanspipe stem powder,get crude extract after rotary evaporation and vacuum dehydration，and dissolved extractum with NaHCO3 saturated solution，adjusted the pH to 3 after delamination.Used silica gel G chromatographic colum to laminar analysis. The eluent was made up of chloroform∶acetidin∶methylbenzol(2∶1∶1).Then get flav-crystal 0.389g after recrystallization, identified and assayed the content with R-HPLC. the result showed that the flav-crystal was aristolochic acid A monomer, and the content reached 99.2%. The methold of extraction could get high purity aristolochic acid A,and it was a high performance way. |
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| Keywords: | manchurian dutchmanspipe stem aristolochic acid A extraction assaying |
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