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| 超级稻DAD1基因的cDNA克隆及其正反义表达载体的构建 | |
| 引用本文： | 蒋泓,周天鸿,洪亚辉,唐冬生,萧浪涛.超级稻DAD1基因的cDNA克隆及其正反义表达载体的构建[J].湖南农业大学学报(自然科学版),2006,32(2):131-134. |
| 作者姓名： | 蒋泓 周天鸿 洪亚辉 唐冬生 萧浪涛 |
| 作者单位： | 1. 暨南大学,生命科学与技术学院,广东,广州,510632;湖南农业大学湖南省植物激素与生长发育重点实验室,湖南,长沙,410128 2. 暨南大学,生命科学与技术学院,广东,广州,510632 3. 湖南农业大学生物科学技术学院,湖南,长沙,410128 4. 湖南农业大学湖南省植物激素与生长发育重点实验室,湖南,长沙,410128 |
| 基金项目： | 湖南省杰出中青年基金项目(02CB014) |
| 摘 要： | 采用RT-PCR方法从超级稻幼苗中扩增DAD1基因的cDNA,并克隆测序,核酸序列表明该基因编码区为342bp,编码114个氨基酸残基.与GenBank发表的序列(XM_472334)相比,有3处碱基不同,分别为105bp处的C(GenBank的为T),148bp处的A(G),149bp处的C(T).将该基因分别正向和反向插入CaMV35S启动子后,构建了基因在植物中的正义、反义表达载体. |
| 关 键 词： | DAD1基因 表达载体 载体构建 超级稻 |
| 文章编号： | 1007-1032(2006)02-0131-04 |
| 修稿时间： | 2006年3月6日 |
Molecular Cloning of Super Hybrid Rice DAD1 and Construction of Plant Expression Vector for Sense and Anti-Sense |
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| JIANG Hong,ZHOU Tian-hong,HONG Ya-hui,TANG Dong-sheng,XIAO Lang-tao.Molecular Cloning of Super Hybrid Rice DAD1 and Construction of Plant Expression Vector for Sense and Anti-Sense[J].Journal of Hunan Agricultural University,2006,32(2):131-134. | |
| Authors: | JIANG Hong ZHOU Tian-hong HONG Ya-hui TANG Dong-sheng XIAO Lang-tao |
| Abstract: | DAD1 cDNA from super hybrid rice(Oryza sativa L.) was amplified by RT-PCR,and the coding sequence of the cDNA was analyzed.The result demonstrated that the coding region of DAD1 cDNA is 342 bp,encodes a protein consisting of 114 amino acids.Compared with the sequence in GenBank,there are three different bases from the gene XM_472334.In order to study the function of this gene,binary vectors were constructed,in which the coding region of the gene was placed under the 35S promoter in either sense or antisense orientation. |
| Keywords: | DAD1 gene plant expressing vector construction super hybrid rice |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |