Leak syndrome of potato in Tunisia caused byPythium aphanidermatum andPythium ultimum |
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| Authors: | M A Triki Sylvie Priou M El Mahjoub A Baudry |
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| Institution: | (1) Laboratoire de Phytopathologie, Ecole Supérieure d'Horticulture et d'Elevage (ESHE), 4042 Chott-Mariem, Tunisia;(2) Present address: Institut National de la Recherche Agronomique de Tunisie/CRPh Degache, 2260 Tozeur, Tunisia;(3) Present address: International Potato Center (CIP), Apartado 1558, Lima 12, Peru;(4) Present address: Laboratoire National de la Protection des Végétaux (LNPV), BP 81, 33883 Villenave d'Ornon cedex, France |
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| Abstract: | Summary TwoPythium spp. were isolated from diseased tubers exhibiting rots at harvest, in summer stores and also in refrigerated storage. Symptoms
shared features described for both pink rot caused byPhytophthora erythroseptica and leak caused byPythium spp.
The causal agents were identified asPythium aphanidermatum andP. ultimum by morphological and physiological observations and by fingerprinting using oomycete specific primers to amplify the internal
transcribed spacer (ITS1) within ribosomal DNAs. The optimum temperatures for infection were 30°C forP. aphanidermatum and 25°C forP. ultimum, corresponding to the optimum temperatures for growth in vitro. The optimum concentration for infection of tubers inoculated
by dipping in a suspension of oospores or hyphal swellings after wounding was found to be 103 reproductive organs/ml, whereas the infection threshold was 10 reproductive organs/ml. |
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| Keywords: | Solanum tuberosum L watery wound rot |
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