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| 猪、鸡大肠杆菌ESBLs的基因型检测 | |
| 引用本文： | 张春辉,张晓风,张晓根,赵传壁,王建华.猪、鸡大肠杆菌ESBLs的基因型检测[J].中国农学通报,2009,25(1):8-11. |
| 作者姓名： | 张春辉 张晓风 张晓根 赵传壁 王建华 |
| 作者单位： | 1. 西北农林科技大学动物医学院,山西杨凌,712100;郑州牧业工程高等专科学校,郑州,450011 2. 郑州牧业工程高等专科学校,郑州,450011 3. 西北农林科技大学动物医学院,山西杨凌,712100 |
| 基金项目： | 河南省教育厅科研项目，河南省科技厅科技攻关项目 |
| 摘 要： | 【目的】为克服产生ESBLs细菌的耐药性，指导临床合理用药。【方法】笔者对产生ESBLs的大肠杆菌，用PCR方法进行了扩增测序和基因型分析【结果】产生ESBLs细菌均扩增出了TEM型引物但都没有扩出SHV引物。【结论】国内猪鸡产ESBLs大肠杆菌目前还未通过质粒进行细菌间耐药基因的交换。 |
| 关 键 词： | 水稻 水稻 蛋白质 可溶性糖 灌浆速率 |
| 收稿时间： | 2008-09-16 |
| 修稿时间： | 2008-11-24 |
Genotype detection of ESBLs |
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| Zhang Chunhui,Zhang Xiaofeng,Zhang Xiaogen,Zhao Chuanbi,Wang Jianhua.Genotype detection of ESBLs[J].Chinese Agricultural Science Bulletin,2009,25(1):8-11. | |
| Authors: | Zhang Chunhui Zhang Xiaofeng Zhang Xiaogen Zhao Chuanbi Wang Jianhua |
| Institution: | Zhang Chunhui, Zhang Xiaofeng, Zhang Xiaogen, Zhao Chuanbi, Wang Jianhua ( Worthwest A ＆F University, Yangling Shanxi 712100; 2Zhengzhou College of A nimal Husbandry Engineering, Zhengzhou 450011) |
| Abstract: | 【Objective】In order to overcome drug resistance of the ESBLs producing bactera ，to instruct the veterinary drug,use in clinic。【Method】 Five Enterobacteriaceaes isolated from swine and chicken , tested for ESBL by double –disk methord, were studied ESBLs gene type with amplified by PCR .【Result】the ESBLs producing bactera amplified by PCR with TEM-type down-object while SHV-type down –object did not.【Conclusion】ESBLs producing Enterobacteriaceaes from swine and avian have not exchanged resistant gene through plasmid in china. |
| Keywords: | ESBLs |
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