| 梨轮纹病菌培养特性研究 |
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| 引用本文： | 张丽丽,常有宏,陈志谊.梨轮纹病菌培养特性研究[J].果树学报,2009,26(4). |
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| 作者姓名： | 张丽丽 常有宏 陈志谊 |
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| 作者单位： | 张丽丽,ZHANG Li-li(南京农业大学园艺学院,南京,210095;江苏省农业科学院植物保护研究所,南京,210014;江苏省农业科学院园艺研究所,南京,210014);常有宏,CHANG You-hong(江苏省农业科学院园艺研究所,南京,210014);陈志谊,CHEN Zhi-yi(江苏省农业科学院植物保护研究所,南京,210014) |
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| 基金项目： | 江苏省农业科技自主创新资金项目 |
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| 摘 要： | 研究了梨轮纹病原菌的培养特性,结果表明,不同病原菌菌株间的致病力、菌丝日生长速率及产孢能力均不同,生长速率最大的菌株为P-16,生长速率最小的菌株为P-10,差异较大。菌株P-16致病力最强,菌株P-10致病力最弱。菌株P-7、P-13、P-16产孢量较大,P-4、P-10、P-19无分生孢子器产生;病原菌菌丝最佳生长温度为28℃,最佳产孢温度为25℃,分生孢子最佳萌发温度28℃;在日光灯、黑光灯条件下有利于病原菌菌丝生长,黑暗条件下病原菌生长较慢;病原菌产孢在黑光灯条件下最好;不同光照条件对病原菌分生孢子萌发影响不大;病原菌菌丝生长最适pH值为7~9,产孢最适pH值为6~8,分生孢子萌发最适pH值为7~9;病原菌菌丝致死温度为62℃(10min)或57℃(15min),分生孢子致死温度为60℃(10min)或55℃(15min)。
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| 关 键 词： | 梨 轮纹病 培养特性 |
Study on the specific method for culturing Botryosphaeria berengeriana f. sp. piricola |
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| ZHANG Li-li,,CHANG You-hong,CHEN Zhi-yi.Study on the specific method for culturing Botryosphaeria berengeriana f. sp. piricola[J].Journal of Fruit Science,2009,26(4). |
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| Authors: | ZHANG Li-li CHANG You-hong CHEN Zhi-yi |
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| Institution: | 1Department of Horticulture;Nanjing Agricultural University;Nanjing;Jiangsu 210095 China;2Plant Protection Institute;Jiangsu Academy of Agricultural Science;Jiangsu 210014 China;3Horticultural Research Institute;Nan- jing;Jiangsu 210014 China |
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| Abstract: | The specific method for culturing Botryosphaeria berengeriana f. sp. piricola was studied. The results showed that different strains had different growth rate,pathogenicity and sporulation quantity. P-16 had the maximum daily growth rate and P-10 had the least; P-16 had the most powerful pathogenicitiy and P-10 had the least; P-7,P-13,P-16 had the largest Sporulation quantity,but P-4,P-10,P-19 had no pycnidium .The optimum temperatures for mycelium growth,sporulation and conidial germination were 28 ℃, 25 ℃ and 28 ℃, respectively. Daylight lamp and Dark light lamp was beneficial to mycelium growth,dark conditions were against mycelium growth; whereas black light lamp condition could promote sporulation. However,the conidial germination showed to be less affected by light conditions. The proper pH values for mycelium growth,sporulation and conidial germination were 7~9, 6~8 and 7~9, respectively. The lethal temperatures for mycelium and conidiospore were 62 ℃ (10 min) or 57 ℃ (15 min), and 60 ℃(10 min ) or 55 ℃(15 min). |
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| Keywords: | Pear Pear ring rot Specific culture |
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