| 一株枯草芽胞杆菌产抑菌物质发酵条件及代谢产物稳定性研究 |
| |
| 引用本文： | 张志焱,程秀芳,刘虹,谷巍.一株枯草芽胞杆菌产抑菌物质发酵条件及代谢产物稳定性研究[J].畜牧与饲料科学,2012,33(3):7-9. |
| |
| 作者姓名： | 张志焱 程秀芳 刘虹 谷巍 |
| |
| 作者单位： | 山东宝来利来生物工程股份有限公司,山东泰安,271000 |
| |
| 摘 要： | 枯草芽胞杆菌L7是从牛粪中选出的,它的代谢产物中的抑菌物质对多种病原菌具有强烈的抑制作用。通过对其抑菌物质产生的发酵条件研究认为,最佳发酵条件为pH值7.0、接种量4%、30℃恒温振荡培养箱培养,发酵时间约60h。其代谢产物中的抑菌物质在80～121℃下30min稳定,pH值在6.0～10.0范围内稳定,光照2～6h后抑菌活性下降。结论：菌株L7的发酵产物具有较强稳定性,可置避光、pH值中性、常温环境中短期保存,经提高活性、纯化等处理后可能作为新型天然防腐剂应用于食品领域,或应用于抗感染微生态制剂的生产。
|
| 关 键 词： | 枯草芽胞杆菌 抑菌物质 发酵条件 代谢产物 稳定性 |
Study on the Fermentation Conditions of Antibacterial Substance Produced by Bacillus subtilis and the Stability of Its Metabolic Products |
| |
| ZHANG Zhi-yan, CHENG Xiu-fang, LIU hong, GU Wei.Study on the Fermentation Conditions of Antibacterial Substance Produced by Bacillus subtilis and the Stability of Its Metabolic Products[J].Animal Husbandry and Feed Science,2012,33(3):7-9. |
| |
| Authors: | ZHANG Zhi-yan CHENG Xiu-fang LIU hong GU Wei |
| |
| Institution: | (Shandong Baolai-leelai Bioengineering Co. Ltd., Tai′an 271000, China) |
| |
| Abstract: | Bacillus subtilis L7 that was isolated from cow dung and the antibacterial substance in its metabolites had strong inhibition on multi-pathogenic bacteria. The studies on the fermentation conditions for producing the antibacterial substance showed that the optimal fermentation conditions were as follows:pH of 7.0, culturing with 4% inoculum amount under constant-temperature oscillation incubator for 60 h at 30 ℃. The antibacterial substance in its metabolites was stable at 80~ 121 ℃ for 30 min under pH of 6.0~10.0. The antibacterial activity decreased under illumination conditions after 2~6 h. In conclusion, the fermented products of strain L7 had stronger stability, so it could be preserved under the environment of lucifuge,neutral pH and room temperature for a short term.And it might be used as a new kind of natural antiseptic in food industry or applied in the production of anti-infection micro -ecological preparation after treatments of increasing the activity and purifying. |
| |
| Keywords: | Bacillus subtilis antibacterial substance fermentation conditions metabolic products stability |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
