| 一株乳酸菌的分离鉴定及生物学特性研究 |
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| 引用本文： | 武香玉,徐海燕,辛国芹,曹银生,谷巍.一株乳酸菌的分离鉴定及生物学特性研究[J].畜牧与饲料科学,2012,33(3):1-3,6. |
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| 作者姓名： | 武香玉 徐海燕 辛国芹 曹银生 谷巍 |
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| 作者单位： | 山东宝来利来生物工程有限公司,山东泰安,271000 |
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| 摘 要： | 以鸡肠道和泡菜为分离源,采用常规微生物学方法分离乳酸菌,筛选到一株产酸性能良好的菌株W7。结合对菌株的形态观察、生理生化试验以及16S rRNA基因序列分析,对菌株进行鉴定,并对W7进行特性试验研究。结果表明,W7为植物乳杆菌（Lactobacillus plantarum）,在培养22h时,活菌数最高;耐酸效果好,在pH值为2.0时,存活率仍然为100%;在胆盐浓度为0.30%时,仍然可以生长;抑菌效果较好;黏附率为79.9%。
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| 关 键 词： | 乳酸菌 分离鉴定 生物学特性 |
Isolation,Identification and Biological Characteristics of Lactic Acid Bacteria |
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| WU Xiang-yu, XU Hai-yan, XIN Guo-qin, CAO Yin-sheng, GU Wei.Isolation,Identification and Biological Characteristics of Lactic Acid Bacteria[J].Animal Husbandry and Feed Science,2012,33(3):1-3,6. |
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| Authors: | WU Xiang-yu XU Hai-yan XIN Guo-qin CAO Yin-sheng GU Wei |
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| Institution: | (Shandong Baolai-leelai Bioengineering Co. Ltd., Tai′an 271000, China) |
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| Abstract: | One strain W7 with high acid production was obtained from the intestinal of chicken and pickle by using the conventional microbiology method. The strain W7 was identified by morphological observation, physiological biochemical test and 16S rRNA gene sequence analysis, and biological characteristics of the strain W7 was studied. Results showed that the strain W7 was identified as Lactobacillus plantarum. The amount of W7 reached the maximum at 37 ℃ for 22 h. The strain W7 had strong resistance to acid, and the survival rate still reached 100% at pH 2.0. The strain W7 could grow when the concentration of bile salt was 0.30%. The strain had good antibacterial effect on some pathogenic bacteria, and the adhesion rate was 79.9%. |
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| Keywords: | lactic acid bacteria isolation and identification biological characteristics |
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