| 几种淡水鱼血清免疫复合物的初步研究 |
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| 引用本文： | 彭宣宪,章跃陵,王三英.几种淡水鱼血清免疫复合物的初步研究[J].中国水产科学,2001,8(3):1-4. |
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| 作者姓名： | 彭宣宪 章跃陵 王三英 |
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| 作者单位： | 厦门大学生命科学学院细胞生物学与肿瘤细胞工程教育部重点实验室 |
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| 基金项目： | 国家自然科学基金资助项目(39770585);IFS基金资助项目(A/2338-2) |
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| 摘 要： | 采用SDS－PAGE技术和免疫酶斑点法（Dot-ELISA),对罗非鱼、草鱼、鲫、镛和鲤5种淡水鱼的血清免疫复合物（IC）进行了研究。结果表明，鲤科的4种鱼之间的电泳条带差异较小，丽鱼科的罗非鱼与鲤科的4种鱼之间的电泳条带有差异但较小。2％PEG能较好地沉淀5种鱼血清IC，且不同鱼血清IC含量有一定的差异。
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| 关 键 词： | 淡水鱼 免疫复合物 SDS－PAGE 免疫酶斑点法 血清 |
| 文章编号： | 1005-8737(2001)03-0001-04 |
| 修稿时间： | 2000年7月24日 |
Circulating immune complexes of some fresh water fishes |
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| PENG Xuan-xian,ZHANG Yue-ling,WANG San-ying.Circulating immune complexes of some fresh water fishes[J].Journal of Fishery Sciences of China,2001,8(3):1-4. |
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| Authors: | PENG Xuan-xian ZHANG Yue-ling WANG San-ying |
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| Abstract: | Circulating immune complexes of Tilapia, Ctenopharyngodon idellus, Carassius auratus, Aristichthys nobilis and Cyprinus carpio were characterized by SDS-PAGE and Dot-ELISA. The results show that the circulating immune complexes of the 5 fishes can be isolated by 4% PEG, and the differences between the 4 fishes of Cyprinidae are not very obvious in the bands of electropherogram, and neither does the difference between Tilapia and the 4 Cyprinidae fishes, but there are some obvious difference between these immune complexes, which may be related to the molecular weight of their antibodies. |
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| Keywords: | fresh water fish immune complex SDS-PAGE Dot-ELISA |
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