| HPLC法测定硫酸头孢喹肟冻干粉针中硫酸头孢喹肟含量 |
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| 引用本文： | 俞吉杰,余祖功,赵杰,刘利锋,段复康,江善祥.HPLC法测定硫酸头孢喹肟冻干粉针中硫酸头孢喹肟含量[J].畜牧与兽医,2008,40(9). |
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| 作者姓名： | 俞吉杰 余祖功 赵杰 刘利锋 段复康 江善祥 |
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| 作者单位： | 南京农业大学动物医学院,江苏,南京,210095 |
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| 摘 要： | 建立高效液相色谱法(HPLC法)测定硫酸头孢喹肟冻干粉针中硫酸头孢喹肟的含量。色谱条件为色谱柱Kromasil C18柱(250 mm×4.6 mm,5μm),以高氯酸钠-磷酸-三乙胺缓冲液(pH3.6):乙腈(体积比90∶10)为流动相,流速为0.8 mL/min,紫外检测波长为254 nm。结果表明,硫酸头孢喹肟在0.05~25μg/mL范围内呈良好的线性关系,r=0.99 96,平均回收率为100.34%(n=9),RSD为1.34%。该方法简便快速,结果准确可靠,可用于硫酸头孢喹肟冻干粉的质量控制研究。
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| 关 键 词： | 硫酸头孢喹肟冻干粉 硫酸头孢喹肟 HPLC |
Determination of Cefquinome sulphate in Cefquinome sulphate lyophilized powder by HPLC |
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| YU Ji-jie,YU Zu-gong,ZHAO Jie,LIU Li-feng,DUAN Fu-kang,JIANG Shan-xiang.Determination of Cefquinome sulphate in Cefquinome sulphate lyophilized powder by HPLC[J].Animal Husbandry & Veterinary Medicine,2008,40(9). |
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| Authors: | YU Ji-jie YU Zu-gong ZHAO Jie LIU Li-feng DUAN Fu-kang JIANG Shan-xiang |
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| Abstract: | This study is to establish HPLC method for the determination of Cefquinome sulphate in Cefquinome sulphate lyophilized powder.The assay was performed on Kromasil C18 column(250 mm×4.6 mm,5 μm)with the buffer solution of sodium perchlorate-H3PO4-triethylamine(pH 3.6)-acetonitrile(V∶V=90∶10)as mobile phase.And the detection wavelength was set at 254 nm.The flow rate was 0.8 mL/min.The results indicated that the Cefquinome sulphate within the range from 0.05 μg/mL to 25 μg/mL was linearly correlated(r=0.999 6),the average recovery was 100.34%(n=9),and the RSD was 1.34%.This method is simple and credible,and can be used for the quality control of the Cefquinome sulphate lyophilized powder. |
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| Keywords: | Cefquinome sulphate lyophilized powder Cefquinome sulphate HPLC |
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