| 以随机扩增多态性DNA(RAPD)技术筛选意大利蜜蜂重要农艺性状遗传标记 |
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| 引用本文： | 孙亮先,吉挺,周斌,陈国宏,袁建军,黄周英.以随机扩增多态性DNA(RAPD)技术筛选意大利蜜蜂重要农艺性状遗传标记[J].中国养蜂,2003,54(6):6-8. |
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| 作者姓名： | 孙亮先 吉挺 周斌 陈国宏 袁建军 黄周英 |
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| 作者单位： | 泉州师范学院 泉州362000
(孙亮先，袁建军)，扬州大学牧医学院 扬州225009
(吉挺，周斌，陈国宏)，泉州师范学院 泉州362000(黄周英) |
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| 基金项目： | 泉州市重点科技项目 (Z2 0 0 0 2 0 )，扬州大学畜牧兽医学院基金项目 ( 2 0 0 3 0 2 2 ) |
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| 摘 要： | 为了获得意大利蜜蜂重要农艺性状的分子遗传标记 ,本研究以 30种RAPD随机引物对平湖浆蜂、美意、澳意、苏意等 4个在产浆、采蜜和抗病性上存在较大差异的意蜂品系的基因组DNA进行RAPD_PCR扩增筛选。试验结果显示 ,随机引物W 1 8(5′_CGGACGGCGG_3′)和W2 3(5′_GTACCGCCCG_3′)的扩增图谱呈多态性。其中 ,在引物W 1 8所扩增出的 9条片断中 ,2 35 2bp和 36 4bp条带为美意所特有 ,表明可将之用于鉴别美意 ;2 0 92bp条带仅出现于蜂蜜高产的美意和澳意中 ,意味着该条带为一个蜂蜜高产性状的遗传标记 ;而 6 32bp条带在王浆高产品系平湖浆蜂中出现的频率 (0 91 )显著高于 (P <0 0 1 )美意(0 0 4 )、澳意 (0 0 2 )和苏意 (0 0 0 ) ,说明 6 32bp条带可能为王浆高产性状的一个遗传标记。在W2 3引物扩增条带中 ,6 5 1bp条带为澳意所特有 ,可用于澳意鉴别
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| 关 键 词： | RAPD 意大利蜜蜂 农艺性状 遗传标记 随机扩增多态性DNA |
| 文章编号： | 0412-4367(2003)06-0006-03 |
| 修稿时间： | 2003年8月26日 |
Screening Genetic Markers of Important Agricultural Traits of Apis mellifera ligustica by Using Random Amplification of Polymorphic DNA (RAPD) |
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| Sun Liangxian ,Ji Ting ,Zhou Bin ,Chen Guohong ,Yuan Jianjun ,Huang Zhouying.Screening Genetic Markers of Important Agricultural Traits of Apis mellifera ligustica by Using Random Amplification of Polymorphic DNA (RAPD)[J].Apiculture of China,2003,54(6):6-8. |
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| Authors: | Sun Liangxian Ji Ting Zhou Bin Chen Guohong Yuan Jianjun Huang Zhouying |
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| Institution: | 1.Quanzhou Normal College; 2.Yangzhou University; |
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| Abstract: | In order to get some molecular genetic markers of important agricultural traits of Italian honey bee, genomic DNA of 4 lines of \%A.m.ligustica\% that with different honey, royal jelly performance and disease resistance capacity (Pinghu royal jelly bee, American_Italian bee, Australia_Italian bee and Jiangsu local Italian bee), were analyzed with RAPD_PCR, After screening 30 RAPD random primers, it was found that primer W18 and W23 could generated polymorphic bands in the 4 lines. In the WI8 primer amplified bands, 2 fragments with the length of 2353 bp and 364 bp were detected only in the American_Italian bees, indicating these 2 markers could be applied in identifying the American_Italian bee; the 2092bp amplified band were detected both in American_Italian and Australia_Italian honey bees, which were characterized with their high performance of honey,but was not detected in the other 2 lines that with relatively lower honey productivity. So it was reasonable to considered this fragment as a DNA marker of high honey productivity trait. The 632 bp amplified band occurred at a higher frequency in the high royal jelly performance variety Pinghu royal jelly bee(0 91) than the other 3 lines (American_Italian bee,0 04; Australia_Italian bee, 0 02; Jiangsu local Italian bee, 0 00), indicting the 632 bp band could serve as a marker of the trait of high royal jelly productivity. In the W23 amplified bands, a 651bp was amplified only in the Australia_Italian bee and this RAPD marker had the potency in the field of varieties identifying. |
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| Keywords: | Apis mellifera ligustica random amplification of polymorphic DNA (RAPD) genetic marker honey royal jelly |
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