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芜菁高频率再生体系的建立及优化
引用本文:马光,周波,李玉花.芜菁高频率再生体系的建立及优化[J].园艺学报,2008,35(6):833-840.
作者姓名:马光  周波  李玉花
作者单位:(东北林业大学生命科学学院,哈尔滨 150040)
基金项目:国家自然科学基金 , 引进国际先进农业科技计划(948计划)  
摘    要:以3个品种芜菁的带柄子叶和胚轴为外植体,采用苯基噻二唑基脲(thidiazuron,TDZ)配合NAA以及BA配合NAA两种组合研究了适于芜菁离体再生的外植体类型和激素组合。发现带柄子叶为外植体,TDZ 7.0 mg·L-1 + NAA 1.0 mg·L-1的组合对不定芽分化最为有利。以此离体再生体系为基础,针对AgNO3浓度、苗龄、2,4-D预培养时间3个因素进行优化,得到了芜菁高频率离体再生体系。结果表明:采用5 d苗龄的带柄子叶作为外植体,在含有2,4-D 1.0 mg·L-1的MS培养基上预培养2 d,添加TDZ 7.0 mg·L-1 + NAA 1.0 mg·L-1 + AgNO3 5.0 mg·L-1的MS培养基为分化培养基对芜菁离体再生最为有利。依品种不同,最高分化率可以达到90%左右。分化后得到的不定芽在IBA 0.1 mg·L-1的MS培养基上可以100%生根,移植成活率达95%。

关 键 词:芜菁  Brassica  rapa  TDZ  硝酸银  再生    
收稿时间:2008-01-03

Establishment and Optimization of a High-frequency Shoot Regeneration System of Turnip (Brassica rapa L. ssp. rapifera)
MA Guang,ZHOU Bo,LI Yu-hua.Establishment and Optimization of a High-frequency Shoot Regeneration System of Turnip (Brassica rapa L. ssp. rapifera)[J].Acta Horticulturae Sinica,2008,35(6):833-840.
Authors:MA Guang  ZHOU Bo  LI Yu-hua
Institution:(College of Life Sciences, Northeast Forestry University, Harbin 150040, China)
Abstract:With cotyledonary petiole and hypocotyl explants, the shoot regeneration frequencies of three turnip (Brassica rapa L. ssp. rapifera) cultivars were examined. To achieve a high-frequency regeneration system, the hormone combination of thidiazuron (TDZ) and naphthaleneacetic acid (NAA) was compared with combination of benzyladenine (BA) and NAA on shoot regeneration. The results show that cotyledonary petioles were the best explant and that Murashige and Skoog (MS) medium containing 7.0 mg·L-1TDZ and 1.0 mg·L-1 NAA was suitable recipe for getting high-frequency shoot regeneration. Based the recipe, the effects of AgNO3 concentration, seedling age, pre-culture time of 2,4-dichlorophenoxyacetic acid (2,4-D) were investigated to optimize the shoot regeneration system. The results suggseted that petiolate cotyledon with seedling age of 5 d cultured in MS medium containing TDZ 7.0 mg·L-1 + NAA 1.0 mg·L-1 + AgNO3 5.0 mg·L-1 followed by pre-culture with 2,4-D 1.0 mg·L-1 for 2d can be induced with highest-frequency regeneration. The highest shoot formation rate was about 90%. The rooting percentage of shoots was 100% on MS supplemented with 0.1 mg·L-1 indole-3-butyric acid (IBA). The 95% rooted shoots survived in a greenhouse.
Keywords:Brassica rapa  TDZ
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