Radioreceptor assay for determination of xylazine and medetomidine in sheep plasma |
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| Authors: | DK MUGE JP CHAMBERS A LIVINGSTON |
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| Institution: | Department of Pharmacology, University of Bristol Medical School University Walk, BS8 1TD, Bristol UK |
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| Abstract: | A radioreceptor assay technique is described for the measurement of xylazine and medetomidine in sheep plasma. The assay was based on the displacement of tritiated clonidine from a 2-adrenoceptors in a rat brain homogenate by xylazine or medetomidine extracted from plasma. Plasma samples from sheep which had been given xylazine and medetomidine were treated with alumina to remove endogenous catecholamines which would otherwise have bound to α2-- adrenoceptors and interfered with the assay. The drugs were then extracted using chloroform, reconstituted in buffer and used to displace 3H]clonidine. The concentration of α2-agonist was calculated by reference to standard curves. The method had a detection limit of 2.5 ng/mL for xylazine and 0.24 ng/mL for medetomidine. The assay could also be used to detect metabolites capable of binding to α2-receptors. |
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