| 牛呼吸道合胞体病毒RT-PCR检测方法的建立 |
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| 引用本文： | 王红,朴范泽,侯喜林.牛呼吸道合胞体病毒RT-PCR检测方法的建立[J].动物医学进展,2008,29(11). |
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| 作者姓名： | 王红 朴范泽 侯喜林 |
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| 作者单位： | 黑龙江八一农垦大学动物科技学院,黑龙江大庆,163319 |
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| 基金项目： | 大庆市科技局资助项目(SGG2006-010) |
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| 摘 要： | 根据GenBankTM上发表的牛呼吸道合胞体病毒核衣壳蛋白N基因序列,设计合成了一对特异性引物,扩增大小为596bp的目的片段,通过特异性试验、敏感性试验和重复性试验建立了牛呼吸道合胞体病毒的RT-PCR检测方法。所建立的牛呼吸道合胞体病毒的RT-PCR方法与牛腺病毒、牛副流感病毒、牛传染性鼻气管炎病毒、牛无浆体均无交叉反应,该方法的敏感性可达1TCID50。结果表明,该方法具有快速、敏感、特异性强和重复性好等特点,可作为牛呼吸道合胞体病毒检测的一种方法。
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| 关 键 词： | 牛呼吸道合胞体病毒 反转录-聚合酶链反应 检测 |
Establishment of RT-PCR for Detection of Bovine respiratory syncytial virus |
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| WANG Hong,PIAO Fan-ze,HOU Xi-lin.Establishment of RT-PCR for Detection of Bovine respiratory syncytial virus[J].Progress In Veterinary Medicine,2008,29(11). |
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| Authors: | WANG Hong PIAO Fan-ze HOU Xi-lin |
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| Institution: | College of Animal Science and Technology;Heilongjiang August-First Land Reclamation University;Daqing;Heilongjiang;163319;China |
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| Abstract: | Specific primers were designed and synthesized according to the reported nucleocapsid (N) protein gene seguence from bovine respiratory syncytial virus (BRSV) in GenBankTM.The size of 596 bp fragment was amplified by different condition optimization,then the RT-PCR diagnostic method of BRSV was constructed by the specific,sensitive and reproducible assays.The diagnostic method of BRSV was no cross-reaction with the closely related clinical symptom pathogen,such as bovine adenovirus,bovine parainfluenza viru... |
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| Keywords: | Bovine respiratory syncytial virus RT-PCR detection |
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