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一株高耐铜真菌的分离鉴定及其在茶园土壤中
引用本文:傅冬和,薛志慧,姚亚丽,曾婷玉,卢向阳.一株高耐铜真菌的分离鉴定及其在茶园土壤中[J].中国农学通报,2012,28(34):268-273.
作者姓名:傅冬和  薛志慧  姚亚丽  曾婷玉  卢向阳
作者单位:1. 国家植物功能成分利用工程技术研究中心,长沙410128;湖南农业大学生物科学技术学院,长沙410128
2. 国家植物功能成分利用工程技术研究中心,长沙,410128
3. 湖南农业大学生物科学技术学院,长沙,410128
基金项目:湖南农业大学博士后基金"富铜富锌微生物的筛选及在环境重金属处理中的应用"
摘    要:为了筛选并鉴定一株耐铜富铜菌株并用于环境中重金属铜的清除,从有一定圈养时间的生猪养殖场周围土壤中,运用微生物筛选的方法广泛分离筛选耐铜微生物,再反复分离纯化筛选目标菌株,对其进行了形态和分子鉴定,在400倍光学显微镜及扫描电镜下进行形态特征观察,并将基于5.8S rDNA通用引物扩增的测序片段在GenBank数据库中使用BLAST工具进行比对。得到10株不同耐铜浓度的真菌菌株,其中耐性最高的菌株A4,其耐铜浓度达到940 mg/L。A4菌在察氏琼脂培养基上生长迅速,综合确定该菌株为烟曲霉(Aspergillus fumigatus)。菌株A4对茶园土壤中的铜有一定吸附作用。

关 键 词:特征  特征  
收稿时间:5/9/2012 12:00:00 AM
修稿时间:2012/8/11 0:00:00

Screen and Identification of High Copper-resisting Fungi and Its’ Application in Absorbing Copper
Fu Donghe , Xue Zhihui , Yao Yali , Zeng Tingyu , Lu Xiangyang.Screen and Identification of High Copper-resisting Fungi and Its’ Application in Absorbing Copper[J].Chinese Agricultural Science Bulletin,2012,28(34):268-273.
Authors:Fu Donghe  Xue Zhihui  Yao Yali  Zeng Tingyu  Lu Xiangyang
Institution:1 National Research Center of Engineering Technology for Utilization of Functional Ingredients from Botanicals,Changsha 410128;2 College of Life Science and Technology,Hunan Agricultural University,Changsha 410128)
Abstract:

The aims were to screen and identify a microorganism strain with absorbing and resisting to copper and to use it removing heavy metal copper in the environment. By using microorganism screening and separating methods, the copper-resisting microorganism were widely obtained from the soil around pig farms with a long captive time. The target microorganism strains were screened and purified repeatedly. Their morphology and molecular identification were analyzed and the microorganism strains were applied to absorb heavy metal copper in tea garden soil. Morphological characteristics were observed under the 400 times optical microscope and scanning electron microscope. Amplification of DNA sequencing fragment based on the 5.8S rDNA universal primers were compared in the GenBank database using the BLAST tool. 10 strains of fungus of different copper-resisting concentrations were separated. The highest concentration of copper-resisting strain of the fungus was A4 which concentration of copper-resisting reaches 940 mg/L. A4 bacteria grew fast in Czapek medium. It was identified synthetically as Aspergillus fumigatus. The strain A4 had good absorption to copper in tea garden soil.

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