| 香蕉冠腐病拮抗细菌B68的诱变选育 |
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| 引用本文： | 林运萍,谭志琼,张荣意.香蕉冠腐病拮抗细菌B68的诱变选育[J].广西农业科学,2007,38(1):45-48. |
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| 作者姓名： | 林运萍 谭志琼 张荣意 |
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| 作者单位： | 华南热带农业大学环境与植物保护学院,海南儋州,571737 |
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| 基金项目： | 华南热带农业大学科技基金资助(Rnd03) |
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| 摘 要： | 以枯草芽孢杆菌B 68为出发菌株,经过紫外线、硫酸二乙酯以及紫外线(UV) 硫酸二乙酯(DES)复合诱变方法,从大量突变株中进行筛选,最后共获得8株优良菌株。其中紫外线诱变获3株,编号为U-28、U-43和U-116,它们对香蕉冠腐病的抑菌效果比原始菌株B 68提高了3.55%、4.37%和3.64%;硫酸二乙酯诱变也获得3株,编号为D-24、D-26、D-87,抑菌效果比原始菌株B 68提高了4.22%、8.48%、3.83%;复合诱变最终获得2株突变株,编号为UD-7,UD-93,抑菌效果比原始菌株B 68提高了2.27%、2.86%。经过传代10代后发现突变株比原始菌株B 68更能保持较高的遗传稳定性。
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| 关 键 词： | 枯草芽孢杆菌B68 香蕉冠腐病 诱变选育 |
| 文章编号： | 1002-8161（2007）01-0045-04 |
| 收稿时间： | 2006-03-29 |
| 修稿时间： | 2006-03-29 |
Study on mutation breeding of antagonistic strain B68 for banana crown rots |
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| LIN Yun-ping,TAN Zhi-qiong,ZHANG Rong-yi.Study on mutation breeding of antagonistic strain B68 for banana crown rots[J].Guangxi Agricultural Sciences,2007,38(1):45-48. |
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| Authors: | LIN Yun-ping TAN Zhi-qiong ZHANG Rong-yi |
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| Institution: | Environment and Plant Protection College, South China University of Tropical Agriculture, Danzhou, Hainan 571737, China |
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| Abstract: | In the experiment,Bacillus subtilis B68 was used as a starting strain,using UV,DES and UV DES mutation methods,a lot of mutants were screened and eight high yield strains were obtained,of which,three were UV radiate mutation strains U-28,U-43 and U-116,three were DES mutation strains D-24,D-26 and D-87,and two were UV DES compound mutation strains UD-7 and UD-93.As compared with B68 strain,the inhibitory effects of U-28,U-43,U-116,D-24,D-26,D-87,UD-7 and UD-93 increased 3.55%,4.37%,3.64%,4.22%,5.90%,3.83%,2.27% and 2.86%,respectively.After ten generations subculture,the mutation strains could keep higher genetic stability as compared to B68. |
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| Keywords: | Bacillus subtilis B68 banana crown rots mutation breeding |
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