| 奶牛乳腺蛋白组分析中蛋白质分步提取方法的建立 |
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| 引用本文： | 秦芝蕾,左谦益,伏小平.奶牛乳腺蛋白组分析中蛋白质分步提取方法的建立[J].动物医学进展,2012(3):66-68. |
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| 作者姓名： | 秦芝蕾 左谦益 伏小平 |
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| 作者单位： | 甘肃农业大学动物医学院;兰州生物制品研究所有限责任公司实验动物室 |
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| 摘 要： | 全息蛋白质样品提取是蛋白组研究的先决条件,采用3种溶解性质不同的裂解液分步提取奶牛乳腺蛋白组,分别进行双向聚丙烯酰胺凝胶电泳分离,PDQUest 7.4分析凝胶图谱,发现其与常规方法相比,具有蛋白质提取率高、双向电泳(2-DE)分辨率高等优点。
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| 关 键 词： | 分步提取 双向电泳 蛋白组 |
Establishment of Sequential Extraction Method in the Proteome Analysis of Mammary Gland from Dairy Cows |
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| QIN Zhi-lei,ZUO Qian-yi,FU Xiao-ping.Establishment of Sequential Extraction Method in the Proteome Analysis of Mammary Gland from Dairy Cows[J].Progress In Veterinary Medicine,2012(3):66-68. |
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| Authors: | QIN Zhi-lei ZUO Qian-yi FU Xiao-ping |
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| Institution: | 1(1.Gansu Agricultural University,Faculty of Veterinary Medicine,Lanzhou,Gansu 730070,China; 2.Lanzhou Institute of Biological Products,Limited corporation,Department of Experimental Animal,Lanzhou,Gansu 730046,China) |
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| Abstract: | The total sample preparation is a prerequisite for proteomic analysis.A three-step sequential protein extraction method was applied in proteomics analysis:using three kinds of solutions with differential solubility to replace one kind of solution.Proteins were separated by IEF and SDS-PAGE.And the patterns were analyzed with PDQUest 7.4 software.The method improved the efficiency of extraction and separation of the proteins compared with method of one solution. |
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| Keywords: | sequential extraction 2-DE proteome |
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