| pH条件对短短芽孢杆菌FJAT-0809-GLX次生代谢物产生的影响 |
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| 引用本文： | 陈峥,刘波,朱育菁,胡桂萍,车建美,唐建阳.pH条件对短短芽孢杆菌FJAT-0809-GLX次生代谢物产生的影响[J].福建农业学报,2012(1):71-76. |
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| 作者姓名： | 陈峥 刘波 朱育菁 胡桂萍 车建美 唐建阳 |
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| 作者单位： | 福建省农业科学院农业生物资源研究所 |
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| 基金项目： | 国家“973”前期项目(2011CB111607);国家“948”项目(2011-G25);福建省青年科技创新基金(2011J05053) |
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| 摘 要： | 应用GC-MS对10种不同初始pH条件下发酵的FJAT-0809-GLX发酵液的丙酮萃取液进行初步成分鉴定,从这10种发酵液中得到匹配率≥90%成分11种,各发酵液中的成分存在明显差异,其中共有成分1种,为六氢吡咯并1,2-a]吡嗪-1,4-二酮。在匹配率≥90%成分中,功能性成分按其功能可分为2类,一类为挥发性或芳香类物质,与该发酵液具有的特殊香气有关,包括5-甲基呋喃醛、2-甲基-3-羟基-4-吡喃酮、苯乙醛、棕榈酸、顺式十八碳-9-烯酸、甲基环戊烯醇酮;另一类为防腐或抑菌类成分,包括2-甲基-3-羟基-4-吡喃酮、2-呋喃甲醇、六氢-吡咯1,2-a]吡嗪-1,4-二酮和5-羟甲基糠醛。初始pH 6～12的发酵液对大肠杆菌K88均有抑制效果,其中初始pH为7的抑菌效果最佳,抑菌圈直径达到20.33mm。不同的初始pH影响着发酵终点pH,进而影响着短短芽孢杆菌FJAT-0809-GLX次生代谢物的产生。
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| 关 键 词： | 次生代谢物 发酵过程 pH 气相色谱质谱联用 短短芽孢杆菌 丙酮萃取 |
Effects of Initial pH Value for the Fermentation of Brevibacillus brevis FJAT-0809-GLX on the Generation of Secondary Metabolites Substances |
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| CHEN Zheng,LIU Bo,ZHU Yu-jing,HU Gui-ping,CHE Jian-mei,TANG Jian-yang.Effects of Initial pH Value for the Fermentation of Brevibacillus brevis FJAT-0809-GLX on the Generation of Secondary Metabolites Substances[J].Fujian Journal of Agricultural Sciences,2012(1):71-76. |
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| Authors: | CHEN Zheng LIU Bo ZHU Yu-jing HU Gui-ping CHE Jian-mei TANG Jian-yang |
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| Institution: | (Institute of Agrobiological Resources,Fujian Academy of Agricultural Sicences,Fuzhou, Fujian 350003,China) |
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| Abstract: | Components of FJAT-0809-GLX fermentation liquid in10 different initial pH values were analyzed by GC-MS.Eleven compounds with the match degree above 90% were identified.The compounds found commonly in these 10 fermentation liquid was Hexahydro-pyrrolo pyrazine-1,4-dione.There were two sorts of functional compounds in the FJAT-0809-GLX fermentation liquids;one is volatile compounds which relate to the special aroma of this fermentation liquid,including 5-methyl-2-Furancarboxaldehyde,Maltol,Benzeneacetaldehyde,n-Hexa-decanoic acid,Oleic Acid,and 2-Hydroxy-3-methyl-2-cyclopenten-1-one.The other sort of functional compound had antiseptic and bacteriostatic effect,including Maltol,2-Furanmethanol and Hexahydro-pyrrolo pyrazine-1,4-dione and 5-(hydroxymethyl)-2-Furancarboxaldehyde.The fermentation liquid with the initial pH value of 6 to 12 had inhibitory activity against K88.The pH value 7 had the best inhibitory activity;the diameter of inhibition zone was 20.33mm.Different initial pH values led to different terminal pH value,and then affect the generation properties of secondary metabolites of Brevibacillus brevis FJAT-0809-GLX. |
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| Keywords: | secondary metabolites fermentation process pH GC/MS Brevibacillus brevis acetone extract |
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