| 铁皮石斛组织培养研究 |
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| 引用本文： | 罗绍强.铁皮石斛组织培养研究[J].广东林业科技,2017,33(6):80-83. |
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| 作者姓名： | 罗绍强 |
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| 作者单位： | 肇庆市国有葵垌林场 |
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| 摘 要： | 以铁皮石斛（Dendrobium catenatum）嫩茎为试验材料，建立组织培养体系。结果表明，铁皮
石斛嫩茎用 HgCl 2 试剂消毒 5~7 min 效果最好，污染率和死亡率极低。最佳诱导培养基为 MS 6-BA1.5
mg/L NAA0.5 mg/L KT0.2 mg/L 3% 蔗糖，诱导率达到 96%。最佳继代培养基是 MS 6-BA1.5 mg/
L NAA0.5 mg/L KT0.2 mg/L 5% 香蕉提取液 5% 水解酪蛋白 3% 蔗糖，增值系数达到 8.0。最适宜铁
皮石斛组培苗生根的培养基为 1/2MS 6-BA0.1 mg/L IBA1.0 mg/L 5% 香蕉提取液 2% 蔗糖，生根率为
97%。移栽 30 d 后，成活率达到 90% 以上。
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| 关 键 词： | 铁皮石斛 外植体 组织培养 |
| 收稿时间： | 2017/6/20 0:00:00 |
| 修稿时间： | 2017/8/11 0:00:00 |
Study on Tissue Culture of Dendrobium catenatum |
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| LUO Shaoqiang.Study on Tissue Culture of Dendrobium catenatum[J].Forestry Science and Technology of Guangdong Province,2017,33(6):80-83. |
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| Authors: | LUO Shaoqiang |
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| Institution: | Kui Dong state-owned forest farm of Zhaoqing,Guangdong,Guangning,526332.China |
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| Abstract: | The tender stem segments of Dendrobium candidum was used for tissue culture and micropropagation. The results showed that 0.1% mercuric chloride solution (5-7min) was appropriate, the pollution and mortality rate was low. The best medium for proliferation was MS 6-BA1.5mg/L NAA0.5mg/L KT0.2mg/L 3%S, the induction rate was 96%. The optimum medium for subculture was MS 6-BA1.5mg/L NAA0.5mg/L KT0.2mg/L 5?nana mud 5% Lactium 3%S, the proliferation coefficient is 8.0, The rooting rate of 97%could be achieved in 1/2MS 6-BA0.1mg/L IBA1.0mg/L 5%Bnana mud 2%S.After 30days, the survival rate was more than 90%. |
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| Keywords: | Dendrobium candidum explants tissue culture |
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