| 睡莲不同器官和不同生长阶段的过氧化物酶和酯酶同工酶比较 |
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| 引用本文： | 张海平,房伟民,陈发棣,丁跃生,杨雪萌.睡莲不同器官和不同生长阶段的过氧化物酶和酯酶同工酶比较[J].浙江林学院学报,2010,27(2):246-250. |
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| 作者姓名： | 张海平 房伟民 陈发棣 丁跃生 杨雪萌 |
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| 作者单位： | 1. 南京农业大学,园艺学院,江苏,南京,210095
2. 江浦艺莲苑花卉有限公司,江苏,南京,211800 |
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| 基金项目： | 上海市农业科技成果及高新技术产业化项目，上海市农业科技攻关项目 |
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| 摘 要： | 利用聚丙烯酰胺垂直板电泳技术对3个睡莲Nymphaea品种‘彼得’‘Peter Slocum’,‘玛珊姑娘’‘Masaniello’和‘得克萨斯’‘Texas Dawn’不同器官（未展开的幼叶、展开的幼叶、老叶、花瓣、花蕾、叶柄）和未展开的幼叶在营养生长阶段和生殖生长阶段的过氧化物酶（POD）和酯酶（EST）同工酶进行了研究。结果表明：①3个品种共有12种过氧化物酶酶带，6种酯酶酶带。②睡莲的过氧化物酶和酯酶具有组织特异性，其中未展开嫩叶和展开嫩叶的酶带最多，叶柄和花蕾次之，花瓣和老叶最少。未展开幼叶为较适合用于睡莲同工酶分析。③与生殖生长阶段相比，3个睡莲品种未展开幼叶在营养生长阶段的酯酶表达量更大，条带更多；而过氧化物酶同工酶表达量则是生殖生长阶段的表达量大，但3个品种的酶条带变化不一致，其中‘彼得’和‘玛珊姑娘’在生殖生长阶段的过氧化物酶同工酶阶段条带多‘得克萨斯’则相反。图4表3参15
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| 关 键 词： | 植物学 睡莲 组织特异性 过氧化物酶（POD） 酯酶（EST） |
Isozyme analysis for different organs and growth stages of water lily |
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| ZHANG Hai-ping,FANG Wei-min,CHEN Fa-di,DING Yue-sheng,YANG Xue-meng.Isozyme analysis for different organs and growth stages of water lily[J].Journal of Zhejiang Forestry College,2010,27(2):246-250. |
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| Authors: | ZHANG Hai-ping FANG Wei-min CHEN Fa-di DING Yue-sheng YANG Xue-meng |
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| Institution: | ZHANG Hai-ping1,FANG Wei-min1,CHEN Fa-di1,DING Yue-sheng2,YANG Xue-meng1(1. College of Horticulture,Nanjing Agricultural University,Nanjing 210095,Jiangsu,China,2. Nanjing Yileen Water Garden Co.,Ltd.,Nanjing 211800,China) |
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| Abstract: | Peroxidase isozyme(POD)and esterase isozyme(EST)of three water lily cultivars,Nymphaea'Peter Slocum',N.'Masaniello',and N.'Texas Dawn',were studied using polyacrylamide gel electrophoresis(PAGE)in different organs(closed young leaf,open young leaf,old leaf,petal,flower bud and petiole)and growth stages of a closed young leaf.Results showed(1)12 POD bands and 6 EST bands.Also,(2)POD and EST were tissue specific for the closed young leaf,open young leaf,old leaf,petal,bud,and petiole.The most isozyme bands were observed in the closed young leaf and the open young leaf with the least in the old leaf and petal.(3)In addition,compared to the reproductive growth stage,the closed leaf in the vegetative growth stage had more EST and EST bands.Meanwhile,there was more POD during the reproductive stage.However,during the reproductive growth stage more POD bands of N.'Peter Slocum'and N.'Masaniello'were found with more POD bands of N.'Texas Dawn'noted during the vegetative growth stage. |
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| Keywords: | botany water lily tissue specificity peroxidase isozyme(POD) esterase isozyme(EST) |
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