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| NaCl胁迫下转入Cu,Zn-SOD基因马铃薯活性氧的代谢及保护酶的变化 | |
| 引用本文： | 刘晓鹏,赵迎春,黄大恩,李卫东,袁勤生.NaCl胁迫下转入Cu,Zn-SOD基因马铃薯活性氧的代谢及保护酶的变化[J].植物保护,2003,29(3):21-24. |
| 作者姓名： | 刘晓鹏 赵迎春 黄大恩 李卫东 袁勤生 |
| 作者单位： | 1. 华东理工大学生物反应器工程国家重点实验室,生物化学研究所,上海,200237;中国南方马铃薯中心,湖北恩施,445000 2. 中国南方马铃薯中心,湖北恩施,445000 3. 华东理工大学生物反应器工程国家重点实验室,生物化学研究所,上海,200237 |
| 摘 要： | 以转入Cu ,Zn-SOD基因的马铃薯为材料 ,研究了在不同NaCl浓度胁迫下其活性氧代谢及保护酶活性的变化。结果表明 ,在NaCl胁迫下转基因马铃薯植株体内的超氧阴离子 (O2-)含量明显低于对照非转基因马铃薯 ,H2O2含量前者略高于后者但差异不大 ;超氧化物歧化酶活性转基因马铃薯显著高于对照 ,过氧化氢酶及抗坏血酸过氧化物酶活性转基因马铃薯高于对照 ;转基因马铃薯体内的脂质过氧化产物丙二醛 (MDA)含量显著低于对照。研究表明 ,转入Cu ,Zn-SOD基因马铃薯具有较强的抗氧化能力 ,具有耐 (抗 )盐的潜力。 |
| 关 键 词： | 基因工程 转基因马铃薯 NaCl胁迫 活性氧代谢 保护酶活性 |
| 文章编号： | 0529-1542(2003)03-0021-04 |
| 修稿时间： | 2002年11月18 |
Metabolism of active oxygen and change of cell defense enzyme in potato transferred with Cu, Zn-SOD gene under NaCl stress |
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| LIU Xiao-peng,; ZhAO Ying-chun,HUANG Da-en,LI Wei-dong,YUAN Qin-sheng.Metabolism of active oxygen and change of cell defense enzyme in potato transferred with Cu, Zn-SOD gene under NaCl stress[J].Plant Protection,2003,29(3):21-24. | |
| Authors: | LIU Xiao-peng ; ZhAO Ying-chun HUANG Da-en LI Wei-dong YUAN Qin-sheng |
| Institution: | 1.State Key Laboratory of Bioreactor Engineering and Institute of Biochemistry, East China University of Science and Technology, Shanghai 200237, China; 2.South Potato Research Center of China, Ensi Hubei 445008, China |
| Abstract: | Metabolism of active oxygen and change of cell defense enzyme in potato transfered with Cu, Zn-SOD gene under NaCl stress were studied. The result showed that the transgenic potato had stronger anti-oxidation ability than non-transgenic potato. With the increase of NaCl concentration, the rate of O - 2 generation in transgenic potato was obviously lower than that of non-transgenic potato; the H 2O 2 content of the former was higher than the latter, but discrepancy not large; the MDA content of the latter was higher than the former apparently. The activities of SOD in transgenic potato were higher than non-transgenic potato. The activities of CAT and APX in transgenic potato were higher than non-transgenic potato. |
| Keywords: | gene engineering transgenic potato NaCl stress activated oxygen cell defense enzyme |
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