| 百合疫病病原菌的鉴定及培养基的筛选 |
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| 引用本文： | 徐秉良,马书智.百合疫病病原菌的鉴定及培养基的筛选[J].植物保护学报,2005,32(3):287-290. |
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| 作者姓名： | 徐秉良 马书智 |
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| 作者单位： | 甘肃农业大学草业学院植物病理系,兰州730070 |
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| 基金项目： | 科技部资助项目，甘肃省兰州市科技局科研项目 |
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| 摘 要： | 从具典型症状的新鲜百合疫病植株茎基部病组织中分离到百合疫霉菌,根据其病原菌菌丝的形态、菌落特征,厚垣孢子、游动孢子囊和卵孢子的形态和大小,以及病原菌致病性测定,该病原菌鉴定为烟草疫霉Phytophthora nicotianae van Brede de Haan.供试的16种培养基中,病原菌在胡萝卜琼脂培养基(CaA)和辣椒琼脂培养基(PeA)上生长最好,生长速率分别为1.771和1.770mm/h.在常规培养条件下,病原菌不易产生厚垣孢子、游动孢子囊和卵孢子,在低温、皮氏溶液和土壤浸出液中分别诱导产生出大量厚垣孢子、游动孢子囊和卵孢子.
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| 关 键 词： | 百合疫病 培养基筛选 致病力测定 |
| 收稿时间： | 2004-08-30 |
| 修稿时间： | 2004年8月30日 |
Screening of media and identification of pathogen causing lily blight |
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| XU Bing-liang and MA,Shu-zhi.Screening of media and identification of pathogen causing lily blight[J].Acta Phytophylacica Sinica,2005,32(3):287-290. |
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| Authors: | XU Bing-liang and MA Shu-zhi |
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| Institution: | Department of Plant Pathology, College of Grassland Sciences, Gansu Agriculture University, Lanzhou 730070, China |
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| Abstract: | The pathogen causing lily blights was isolated from diseased lily with typical symptoms on bas- al stem.The pathogen was identified as Phytophthora nicotianae van Brede de Haan according to the characteristics of mycelium,chlamydospore,zoosporangium,oospore and the test of pathogenicity of the pathogen.Among 16 kinds of culture media,the best media for pathogen growing and generating were carrot agar medium (CaA) and pepper agar medium (PeA) with growth speed 1.771 mm/h and 1.770mm/h respectively.It is hard for Phytophthora nicotianae to produce chlamydospores,zoosporangi- ums and oospores under normal culture conditions.With the treatment of low temperature,Petri solution and filtered soil suspension,pathogen could generate plenty of chlamydospores,zoosporangiums and oos- pores. |
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| Keywords: | Phytophthyora nicotianae screening of medium test of pathogenicity |
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