| 不同方法提取的柱状嗜纤维菌脂多糖对鲤免疫活性的比较 |
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| 引用本文： | 陈昌福.不同方法提取的柱状嗜纤维菌脂多糖对鲤免疫活性的比较[J].华中农业大学学报,1997,16(4):380-385. |
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| 作者姓名： | 陈昌福 |
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| 作者单位： | 华中农业大学水产学院 |
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| 基金项目： | 国家和湖北省自然科学基金 |
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| 摘 要： | 采用不同方法提取的柱状嗜纤维菌脂多糖（ＬＰＳ）作为抗原、对鲤注射免疫后，通过测定受免鲤血清中凝集抗体效价、沉降抗体、白细胞吞噬活性和进行强毒攻击的方法，比较了不同方法提取ＬＰＳ的免疫原性，结果表明，Ｂｏｉｖｉｎ法和Ｐｈｅｎｏｌ－ｗａｔｅｒ法较ＥＤＴＡ法提取ＬＰＳ的免疫原性强，而Ｂｉｏｖｉｎ法和Ｐｈｅｎｏｌ－ｗａｔｅｒ法提取ＬＰＳ的免疫原性则没有明显差别。
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| 关 键 词： | 鲤鱼 柱状嗜纤维菌 脂多糖 免疫应答 |
COMPARISON OF IMMUNOGENICITY OF LIPOPOLYS ACCHARIDE EXTRACTED FROM Cytophaga columnaris BY DIFFERENT METHODS ON CARP |
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| Chen Changfu.COMPARISON OF IMMUNOGENICITY OF LIPOPOLYS ACCHARIDE EXTRACTED FROM Cytophaga columnaris BY DIFFERENT METHODS ON CARP[J].Journal of Huazhong Agricultural University,1997,16(4):380-385. |
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| Authors: | Chen Changfu |
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| Abstract: | Carp ( Cyprinus carpio ) was immunized by injection with lipopolysaccharide (LPS) extracted by different methods. The immunogenicity of differently prepared LPS was compared by examining the agglutinating antibody titer, precipitating antibody and phagocytic activity of leucocytes and challenge test with live Cytophaga columnars . The results whowed that the immunogenicity of LPS extracted by Biovin and Phenolwater method was higher than that extracted by EDTA method while there was no singificant difference bwtween the former to methods. |
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| Keywords: | carp Cytophaga columnaris lipopolysaccharide (LPS) immune response |
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