| 利用改进的一步法提取本地毛形线虫总RNA |
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| 引用本文： | 郑宝亮,付守申,郜相君,马辉,郑鸣,赵绪永,郭宏伟,杨向科.利用改进的一步法提取本地毛形线虫总RNA[J].郑州牧业工程高等专科学校学报,2007,27(2):1-2,5. |
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| 作者姓名： | 郑宝亮 付守申 郜相君 马辉 郑鸣 赵绪永 郭宏伟 杨向科 |
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| 作者单位： | 1. 郑州牧业工程高等专科学校,生物工程系,河南,郑州,450011
2. 大庆油田物业集团,黑龙江,大庆,163453 |
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| 基金项目： | 河南省科技厅科技攻关项目 |
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| 摘 要： | 利用胃蛋白酶消化小鼠肌肉,获取了旋毛虫种本地毛形线虫(T.nativa)的肌幼虫虫体。利用TRIZOL混合虫体直接提取肌幼虫的总RNA,通过分光光度计Ultrospec 3000检测结果,以及用RT-PCR方法扩增出了编码T.na- tiva 49 kDa的ES蛋白结构基因,后续试验结果显示改进的一步法提取本地毛形线虫总RNA效果很理想。
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| 关 键 词： | 肌幼虫 总RNA |
| 文章编号： | 1008-3111（2007）02-0001-02 |
| 修稿时间： | 2007-01-22 |
An improved single-step method for RNA isolation of Trichinella nativa |
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| ZHENG Bao-liang,FU Shou-shen,GAO Xiang-jun,MA Hui,ZHENG Ming,ZHAO Xu-yong,GUO Hong-wei,YANG Xiang-ke.An improved single-step method for RNA isolation of Trichinella nativa[J].Journal of Zhengzhou College of Animal Husbandry Engineering,2007,27(2):1-2,5. |
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| Authors: | ZHENG Bao-liang FU Shou-shen GAO Xiang-jun MA Hui ZHENG Ming ZHAO Xu-yong GUO Hong-wei YANG Xiang-ke |
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| Institution: | 1. Bioengineering Department of Zhengzhou College of Animal Husbandry and Engineering, Zhengzhou Henan 450011, China; 2. Property management group of Daqing oil field, Daqing H eilongjiang 163453, China. |
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| Abstract: | The muscle-stage larvae of Trichinella nativa (T.nativa) were collected digested by pepsin-HCI.The to- tal RNA of T.nativa was isolated using TRIZOL according to the manufactures instruction after the worms being pes- tled together with TRIZOL.It was showed that from the results of RNA detection with Ultrospec 3000,and also from the next result that the gene of the worm which encoding 49kDa ES protein of T.nativa was amplified by RT-PCR, that the improved single-step method of RNA isolation for T.nativa we used was ideal. |
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| Keywords: | Trichinella nativa |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
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