| 浅析荧光PCR检测出现假阳性和假阴性的原因 |
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| 引用本文： | 黄露菡,杨瑞龙,郑妍.浅析荧光PCR检测出现假阳性和假阴性的原因[J].广东畜牧兽医科技,2014,39(6):50-51. |
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| 作者姓名： | 黄露菡 杨瑞龙 郑妍 |
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| 作者单位： | 潮州市动物卫生监督所,广东潮州,521000 |
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| 摘 要： | 荧光PCR技术的高敏感性导致检测结果易出现假阳性和假阴性。阳性样本和阳性对照污染是导致假阳性的原因;RNA酶降解核酸是导致假阴性的重要原因。避免出现假阳性和假阴性检测结果需从实验室设置和管理、操作人员和试验器材管控入手。
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| 关 键 词： | 荧光PCR 假阳性 假阴性 |
Causes of False Positive and False Negative in Fluorescence PCR Detection |
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| Huang Luhan,Yang Ruilong,Zheng Yan.Causes of False Positive and False Negative in Fluorescence PCR Detection[J].Guangdong Journal of Animal and Veterinary Science,2014,39(6):50-51. |
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| Authors: | Huang Luhan Yang Ruilong Zheng Yan |
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| Institution: | ( Chaozhou Animal Health Supervision, Chaozhou 521000,China ) |
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| Abstract: | The high sensitivity of fluorescence PCR is easily lead to false positive and false negative. False positive is due to pollution from positive samples and the positive control. Rnase degradation is an important factor of false negative. False positive and false negative is avoided by improved laboratory setup and management, operating personnel and test equipment control. |
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| Keywords: | Fluorescence PCR false positive false negative |
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