| 荔枝胚性悬浮培养物的建立,保持和优化原生质体分离的研究 |
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| 引用本文： | 俞长河,陈振光.荔枝胚性悬浮培养物的建立,保持和优化原生质体分离的研究[J].热带作物学报,1998,19(3):16-20. |
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| 作者姓名： | 俞长河 陈振光 |
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| 作者单位： | 福建农业大学园艺系 |
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| 摘 要： | 将松散颗粒状的荔枝胚性愈伤组织转入液体培养基，２－３周后即可建立起优质的悬浮培养物，长时间悬浮培养后，荔权胚性悬浮培养物的生长势和分化能力下降，但液体培养基＋固体培养基交替培养可使其保持稳定良好的状态。材料来源，继代时间，酶液浓度，酶液渗透压和高渗预处理对胚性悬浮培养物的原生质体分离均有明显影响。
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| 关 键 词： | 荔枝 胚性悬浮培养物 原生质体分离 预高渗处理 |
Embryogenic Suspension Culture and Protoplast Islolation in Litchee |
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| Yu Changhe,Chen Zhenguang.Embryogenic Suspension Culture and Protoplast Islolation in Litchee[J].Chinese Journal of Tropical Crops,1998,19(3):16-20. |
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| Authors: | Yu Changhe Chen Zhenguang |
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| Abstract: | Friable,granular embryogenic calli of litchee (Litchi chinensis Sonn ) were transferred into liquid medium for culture,and suspension cultures were established after two to three weeks of culture. Long -term cultures (more than ten subcultures) resulted in reduced growth and embryogenic capacity, whereas alternative culture with liquid medium (six subcultures )and solid medium (one subculture )kept the suspension cultures in a good condition. Sources of embryogenic suspension cultures,subculture duration,concentrations of both enzyme and mannitol in isolation solution,and proplasmolyzing all had significant effects on protoplast isolation from embryogenic suspension cultures. The optimum process for isolating of protoplasts was developed. The 4th -day cells used were proplasmolyzed in 13 % mannitol for one hour,then macerated in enzyme solution containing 0. 8 % calllase R - 10, 0. 4 % Macerease R - 10and 10 % or 11 % mannitol. Protoplasts were repetitively isolated at a yield of over 107/gfw and viability of 92 % or more under the optimum conditions. |
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| Keywords: | litchee embryogenic suspension culture protoplast isolation proplasmolzing |
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