| 木霉对多菌灵的生物降解特性研究 |
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| 引用本文： | 田连生,陈菲.木霉对多菌灵的生物降解特性研究[J].土壤学报,2009,46(6):1127-1131. |
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| 作者姓名： | 田连生 陈菲 |
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| 作者单位： | 1. 扬州工业职业技术学院化工系,江苏扬州,225127
2. 扬州工业职业技术学院基础部,江苏扬州,225127 |
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| 摘 要： | 从耐药性木霉菌株的诱变选育过程中,得到一株能在含多菌灵2 000 mg L-1培养基上生长的变异菌株T32。该菌株在以多菌灵为唯一碳源的无机盐培养基中,于25℃、200 rm in-1振荡培养5 d,对多菌灵的降解率达到61.4%。在pH6.0、25℃、5%接种量和加入0.5%酵母粉为最适降解条件下,该菌株对多菌灵、速克灵、扑海因、甲基布托津和三唑酮这5种常用化学农药的降解率分别达到91.4%、92.1%、55.3%、40.1%和86.5%。对原土壤、自然风干土壤和高温烘干土壤中的多菌灵进行室内降解实验,在25~28℃、5%接种量、含水量维持15%的条件下处理10 d,对多菌灵的降解率分别达到78.6%、75.3%和70.5%。
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| 关 键 词： | 多菌灵 木霉 生物修复 变异菌株 |
Carbendazim biodegradation characteristics of Trichoderma |
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| Tian Liansheng and Chen Fei.Carbendazim biodegradation characteristics of Trichoderma[J].Acta Pedologica Sinica,2009,46(6):1127-1131. |
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| Authors: | Tian Liansheng and Chen Fei |
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| Institution: | Chemical Engineering Department, Yangzhou Polytechnic Institute;Fundamental Courses Department, Yangzhou Polytechnic Institute |
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| Abstract: | A Trichoderma mutant strain T32, which can grow on the carbendazim containing culture medium was isolated out of pesticide tolerant Trichoderma strains. Its carbendazim degrading rate reached 61.4% in the mineral medium that utilizes carbendazim as the sole carbon source after 5 days of shake at temperature 25℃ and 200 r min~(-1). In the medium 6.0 in pH value, 25℃ in temperature, 5% in inoculation rate and 0.5% in yeast addition rate, its degrading rates of five common chemical pesticides - carbendazim, procymidone, iprodione, thiophanate-methyl and triadimefon reached 91.4%, 92.1%, 55.3%, 40.1% and 86.5% respectively, demonstrating that Strain T32 has a relatively extensive degradation capacity. Trichoderma can degrade five different kinds of chemical pesticides including carbendazim at the same time, which has not yet been involved in the present research on degrading bacteria. An indoor test of degrading carbendazim in original soil, natural air-drying soil and high-temperature oven-drying soil for 10 days revealed that their carbendazim degrading rate was 78.6%,75.3% and 70.5%, respectively, when the optimal degradation conditions were 25～28℃ in temperature, 5% in inoculation rate, 50 mg g~(-1) in concentration of carbendazim and 15% in water content. The findings may serve as a scientific basis for applying Strain T32 to bioremediation of polluted soil. |
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| Keywords: | Carbendazim Trichoderma Bioremediation Mutant strain |
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