| 人参果组织培养技术研究 |
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| 引用本文： | 张俊伟,郭盘江,陈冠吉.人参果组织培养技术研究[J].西南林学院学报,2003,23(2):21-22. |
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| 作者姓名： | 张俊伟 郭盘江 陈冠吉 |
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| 作者单位： | 西南林学院,资源学院,云南,昆明,650224 |
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| 基金项目： | 云南省省级重点建设专业西南林学院林学专业资助 |
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| 摘 要： | 以人参果的幼嫩茎段和叶片作外植体，在MS基本培养基附加不同浓度6-BA IAA,6-BA NAA 和 KT 2，4 D 组成的固体培养基中进行培养．结果显示：MS 6-BA 0．07 mg／L IAA 0.03mg／L，MS 6-BA 0.70mg／L IAA 0.03mg／L，MS 6-BA 0.70mg／L IAA O.05mg/L，MS 6-BA 1.00mg/L IAA 0.05mg/L 4种培养基中的材料愈伤组织长势比较好，分化明显、经继代培养，丛生芽大量形成，丛生苗根系发达、其他培养基则停留在愈伤组织阶段或只长出极少数短的根.
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| 关 键 词： | 人参果 组织培养 幼嫩茎段 叶片 外植体 培养基 |
| 文章编号： | 1003-7179(2003)02-0021-02 |
A Study on Culture Technology of Tissue of Solanum muricatum |
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| ZHANG Jun-wei,GUO Pan-jiang,CHEN Guan-ji.A Study on Culture Technology of Tissue of Solanum muricatum[J].Journal of Southwest Forestry College,2003,23(2):21-22. |
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| Authors: | ZHANG Jun-wei GUO Pan-jiang CHEN Guan-ji |
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| Abstract: | The delicate stem cuttings of Solanum muricatum. were inoculated on MS base medium supplied with different concentrations of 6-BA and IAA. The results showed that, the differentiation of callus on the MS+6-BA 0.07mg/L+IAA 0.03 mg/L, MS+6-BA 0.70 mg/L + IAA 0.03 mg/L,MS+6-BA 0.70 mg/L+IAA 0.05 mg/L,MS+6-BA 1.00 mg/L+IAA 0.05 mg/L were obvious. By continuing culture, the number of roots increased and grew well.The other stem cuttings were difficult to induced to callus or few roots were induced |
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| Keywords: | Solanum muricatum tissue culture callus |
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