Resistance of E.coli from Fur-bearing Animals to Fluoroquinolones and Compound Chinese Medicine |
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| Authors: | YU Jie QU Hai-na ZHANG Can LI Wen-li REN Hui-ying |
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| Institution: | 1. College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, China;
2. Pingdu Occupation Secondary Specialized School, Pingdu 266700, China |
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| Abstract: | The aim of this study was to investigate the resistance to fluoroquinolones and compound Chinese medicine, and control colibacillosis in fur-bearing animals.14 E.coli strains isolated from diseased fur-bearing animals were detected by PCR for the fluoroquinolone-resistant gyrA gene,and then the PCR fragment of gyrA gene was cloned,sequenced and analyzed for homology.Afterwards,the fragment was labeled with digoxigenin as DNA probe for dot-blot hybridization detection.The resistance to compound Chinese medicine was detected by Oxford-cup tests.The results demonstrated that the positive rates of PCR and dot-blot hybridization were 57.1% and 50.0%,respectively.Only 28.6% of the E.coli strains were resistant to compound Chinese medicine.The results indicated that the resistance to fluoroquinolones in E.coli strains from fur-bearing animals was severe,however,the isolates were much more sensitive to compound Chinese medicine.The results would provide basis for control of colibacillosis of fur-bearing animals. |
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| Keywords: | fur-bearing animals E coli fluoroquinolone compound Chinese medicine antibioic resistance |
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