Effect of Heat Shock Protein-90 (HSP-90) mRNA Expression in Intestinal Tissuse of Chickens Infected with E.necatrix |
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| Authors: | HOU Ning LV Zhi-hui ZHAO Liang GAO Xue-li LIU Chao-nan LV Xiao-ping ZHENG Shi-min |
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| Institution: | 1. College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China;2. Zhuhai Campus of Zunyi Medical College, Zhuhai 519041, China |
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| Abstract: | The 90 chickens were randomly divided into control group, E.necatrix one time infection group and E.necatrix two times infection group.We used duplex PCR method to test the expression changes of HSP-90 mRNA in intestinal tissue of chickens infected with E.necatrix and researched the effects of it comprehensively and systematically.The results were showed that after 0 to 14 days the expression of HSP-90 mRNA in intestinal tissues of 14-day-old chickens infected with E.necatrix presented a upward trend though it was lower than the control chickens.These chickens were infected with E.necatrix again at 28 days, HSP-90 mRNA expressions of duodenum, jejunum and ileum decreased and then increased.However, it always showed a downward trend in appendix.When 28 days chickens were infected with high-does E.necatrix once, HSP-90 mRNA expressions of duodenum, jejunum and appendix were in a declining curve.While it was increased originally and then decreased in ileum.This study indicated that HSP-90 mRNA expressions in intestinal tissue of chickens infected with E.necatrix were inhibited at first, and the expression quantity was closely related to the degrees of intestinal tissue damage. |
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| Keywords: | E necatrix chicken intestinal tissue HSP-90 mRNA expression |
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