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黄瓜上甜瓜黄斑病毒寿光分离物的初步鉴定及序列分析
引用本文:乔 宁,王兴翠,田素波,刘永光,姜会霞,李美芹,竺晓平.黄瓜上甜瓜黄斑病毒寿光分离物的初步鉴定及序列分析[J].中国蔬菜,2015,1(7):25.
作者姓名:乔 宁  王兴翠  田素波  刘永光  姜会霞  李美芹  竺晓平
作者单位:(;1.潍坊科技学院,山东寿光262700;;2. 山东寿光蔬菜种业集团有限公司,山东寿光262700;;3. 山东农业大学植物保护学院,山东泰安271018)
基金项目:公益性行业(农业)科研专项(201303028),山东省农业良种工程现代种业企业科技培育计划项目,山东省自然科学基金项目(ZR2012CM032),山东省科技发展计划项目(2010GNC10915),潍坊市科技发展计划项目(201301159,2013YD177),潍坊科技学院校级项目(W13K033,W13K006)
摘    要:在山东寿光黄瓜保护地种植区采集到疑似感染甜瓜黄斑病毒(Melon yellow spot virus,MYSV)的黄瓜病叶,采用RT-PCR对其进行检测,将扩增产物连接到pEASY-T1 Simple克隆载体后进行测序,结果显示目的片段的大小为505bp,与预期结果一致;序列同源性比对分析结果表明,黄瓜上MYSV寿光分离物与中国三亚的MYSV-Sanya分离物(甜瓜-GQ397254)和中国台湾MYSV-TW(西瓜-FJ386391)亲缘关系较近,同源性可达到98%。

关 键 词:甜瓜黄斑病毒  黄瓜  RT-PCR  序列分析  

Initial Identification and Sequence Analysis of Melon yellow spot virus on Cucumber in Shouguang Isolates
QIAO  Ning,WANG Xing-Cui,TIAN Su-Bo,LIU Yong-Guang,JIANG Hui-Xia,LI Mei-Qin,ZHU Xiao-Ping.Initial Identification and Sequence Analysis of Melon yellow spot virus on Cucumber in Shouguang Isolates[J].China Vegetables,2015,1(7):25.
Authors:QIAO  Ning  WANG Xing-Cui  TIAN Su-Bo  LIU Yong-Guang  JIANG Hui-Xia  LI Mei-Qin  ZHU Xiao-Ping
Institution:(;1.Weifang University of Science and Technology, Shouguang 262700,Shandong,China;;2.Shandong Shouguang Vegetable-Seed Industrial Group, Co. Ltd, Shouguang 262700, Shandong,China;;3.College of Plant Protection, Shandong Agricultural University, Taian 271018, Shandong,China)
Abstract:Cucumber leaves infected possibly by Melon yellow spot virus(MYSV) were collected from Shouguang, Shandong Province. These samples were detected by RT-PCR. The amplified products were connected to pEASY -T1 Simple cloning vector and then sequencing was conducted. The results showed that the size of target fragment was 505 bp, which was consistent with the expected result. And homology analysis revealed that
the MYSV infected cucumber in Shouguang had closer genetic relationship with MYSV-Sanya isolates( melon -GQ397254) from Sanya,China and MYSV-TW isolates( watermelon -FJ386391) from Taiwan, China. Their homology could reach 98%.
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