Isolation,Identification and Pathogenicity Research of a Strain of Clostridium perfringens from Goat |
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| Authors: | MENG Xia SUN Cui-ping TANG Na MIAO Li-zhong SHEN Zhi-qiang |
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| Institution: | 1. Shandong Lvdu Bio-science and Technology Co., Ltd., Binzhou 256600, China;2. Shandong Binzhou Animal Science and Veterinary Medicine Academy, Binzhou 256600, China |
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| Abstract: | A pathogenic bacteria was isolated from a dead goat,and it was identified as goat-pathogenic type A Clostridium perfringens by differential culture,biochemical test,molecular biological technology and animal pathogenicity test.Analysis results of toxin genes showed that this bacteria contained both α and β2 toxin genes.Animal pathogenicity test result showed that this bacteria was highly pathogenic to mice,and the same bacteria was isolated from dead mice as which was isolated from the dead goat,so it was certain that type A Clostridium perfringens was the main pathogenic bacteria which caused the goat dead.The results would provide scientific basis for the treatment and prevention of the disease caused by Clostridium perfringens in this goat farm. |
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| Keywords: | goat Clostridium perfringens isolation anaerobic cultivation bacteriological identification |
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