Effect of MCPH1 on irradiation induced DNA damage in esophageal cancer cells |
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| Authors: | L&# Xin-rui WANG Bao-fang WANG Bao-hui XU Xiao CHEN Ming-liang |
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| Institution: | 1. Department of Physiology, Medical College of Henan University, Kaifeng 475004, China;
2. Department of Neurology, Kaifeng Children Hospital, Kaifeng 475004, China |
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| Abstract: | AIM: To discover the effect of MCPH1 on the DNA damage induced by ionizing radiation in esophageal cancer cells. METHODS: ECA109 cancer cells were radiated at dose of 8 Gy. The nuclear foci of relevant factors were detected 1 h after irradiation in the ECA109 cells after silence of MDC 1 gene. A cell line was established that was stable low expression of MCPH 1 . The nuclear foci induced by ionizing radiation after silence of MCPH 1 were determined. RESULTS: The MCPH1 gene silenced ECA109 cell line was successfully constructed. A strong relationship between MDC1, MCPH1 and γ-H2AX was observed 1 h after 8 Gy irradiation. Silence of MDC 1 did not affect the nuclear foci formation of γ-H2AX and MCPH1. The nuclear foci of MDC1 but not γ-H2AX significantly reduced after silencing of MCPH 1 . CONCLUSION: MCPH1 is located in the downstream of H2AX and upstream formation of MDC1, and regulates the nuclear foci formation of MDC1 during DNA damage response. |
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| Keywords: | MCPH1 Irradiation MDC1 DNA double-strand breaks |
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