Development of Loop-mediated Isothermal Amplification Assay for Detection of Torque teno sus Virus Type 2 |
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| Authors: | ZHANG Li LI Kai TIAN Guang-ling LI De-feng WANG Wei-song HE Hou-jun |
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| Institution: | College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China |
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| Abstract: | In order to develop a loop-mediated isothermal amplification (LAMP) assay for rapid and specific detection of Torque teno sus virus type 2 (TTSuV2), two pairs of primers were designed according to the untranslated regions and the part of open reading frame 1 of TTSuV2.The LAMP system was optimized by adjusting the concentrations of some components and reaction conditions.The optimized amplification conditions of LAMP assay was at 64 ℃ for 90 min.The results showed the LAMP assay was specific for TTSuV2 detection, which could achieve a detection limit of 100 copies/μL viral nucleic acid, and no cross-reaction with TTSuV1, PCV2, CSFV, PRRSV and PBoV.In conclusion, this assay was a rapid, specific and sensitive detection technique which could provide a assistance for the rapid detection of TTSuV2. |
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| Keywords: | Torque teno sus virus type 2 (TTSuV2) loop-mediated isothermal amplification (LAMP) detection |
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