Cloning and Sequence Analysis of gE and gI Genes of Pseudorabies Virus NP Isolate |
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| Authors: | LIN Qun-qun ZHENG Xiao-xiang CHEN Peng-qiang CHEN Qiu-yong ZENG Xian-cheng HU Chong-wei XIU Jin-sheng |
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| Institution: | College of Animal Sciences, Fujian Agricultural and Forestry University, Fuzhou 350002, China |
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| Abstract: | According to published gE and gI gene sequences of pseudorabies virus (PRV) in GenBank, we designed two pairs of primers for PCR amplification of gE and gI genes of PRV NP isolate, after PCR products recycling, cloning and sequencing, the sequencing results were consistent with expectations of PRV gE and gI genes.Homology comparison analysis results revealed that compared with the domestic PRV strains, the homologies of gE and gI amino acids of PRV NP isolate were 95.7% to 99.8% and 89.9% to 99.5%, respectively.Phyogenetic tree analysis and amino acid sequence alignment results found that the gE amino acid sequence site changes of PRV NP isolate were the same with the PRV strains which were isolated from domestic in 2012, thus we could speculate that PRV NP isolate had mutanted.This study had laid the foundation for the epidemiological investigation and analysis of PRV, also provided the scientific basis for the development of scientific, effective and new pseudorabies vaccine. |
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| Keywords: | pseudorabies virus (PRV) gE gene gI gene sequence analysis |
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