Establishment and Preliminary Application of Nested PCR for the Detection of Porcine Circovirus Type 2 |
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| Authors: | YU Jing CHEN Yan-yong HE Xiao-jiang DAI Bing ZHAO A-yong WANG Xiao-du SONG Hou-hui |
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| Institution: | College of Animal Science and Technology, Zhejiang Agriculture & Forestry University, Lin'an 311300, China |
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| Abstract: | Porcine circovirus type 2 (PCV2) caused significant economic losses of swine industry,it's very important to establish a economy and rapid detection method.To strengthen the prevention and control of porcine circovirus disease,rapid and high sesitive detection method of PCV2 was established using nested PCR.According to PCV2 ORF2 gene conservative region,we designed two pairs of nested PCR primers,constructed ORF2 gene recombinant plasmid for standard template and optimizated the PCR reaction conditions to establish nested PCR detection method of PCV2 and detect the clinical samples of pig farms.Our results showed that the sensitivity and repeatability of this method were very high,the minimum detection level was 10 copies/μL and detection rate of clinical samples was 97.3%.In brief,the established nested PCR detection method of PCV2 in this study provided powerful means for quick and efficient detection of PCV2,and prevention and control of porcine circovirus disease in the swine industry. |
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| Keywords: | porcine porcine circovirus type 2 (PCV2) nested PCR |
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