Expression of Gab2 in human osteosarcoma U2-OS cells |
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| Authors: | PING Yong DI Ping-shan LI Wei-bing LI Feng-mei CHEN Wei-yi CHEN Ping-ping |
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| Institution: | 1. Department of Orthopaedics, The People's Hospital of Donggang Region, Rizhao 276800, China;
2. Emergency Department, The People's Hospital of Donggang Region, Rizhao 276800, China;
3. Department of Cardiology, The People's Hospital of Wulian Region, Rizhao 262300, China;
4. Department of Pathology, Weifang Medical University, Weifang 261053, China |
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| Abstract: | AIM: To investigate the expression of Grb2-associated binding protein 2 (Gab2) in human osteosarcoma cells and its relationship with the invasion and metastases of human osteosarcoma cells. METHODS: The technique of small RNA interference was used to transfect human osteosarcoma U2-OS cell lines. Western blotting and RT-PCR were used to detect the protein and mRNA expression of Gab2 in transfected U2-OS cells. After transfection, through chemotaxis and invasion assays in vitro, the cell migration and invasion abilities were detected. RESULTS: After transfection, the expression of Gab2 at mRNA and protein levels in Gab2 siRNA transfected cells (SiGab2/U2-OS) was lower than that in scrambled siRNA transfected cells (Scr/U2-OS) and U2-OS cells. After stimulation with epidermal growth factor (EGF) at concentration of 10 μg/L, the migration SiGab2/U2-OS cells was significantly less than Scr/U2-OS cells and U2-OS cells (P<0.01). The number of invasion cells of SiGab2/U2-OS group was significantly lower than the other 2 control groups (P<0.01). CONCLUSION: Inhibition of Gab2 expression obviously attenuates the migration and invasion abilities of human osteosarcoma U2-OS cell line. |
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| Keywords: | Grb2-associated binding protein 2 Small interference RNA Cell invasion U2-OS cells |
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