Effects of phycocyanin on apoptosis of human laryngeal cancer HEP-2 cells |
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| Authors: | YING Jun PAN Ruo-wang WANG Mao-feng CHEN Ji-shun LIU Qian ZHANG Hong-qin BAO Qi-yu LI Pei-zhen |
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| Institution: | 1. School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou 325035, China;
2. 118 Hospital of PLA, Wenzhou 325000, China;
3. Affiliated Dongyang Hospital of Wenzhou Medical University, Dongyang 322103, China |
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| Abstract: | AIM: To investigate the effect of phycocyanin on the apoptosis of human laryngeal cancer HEP-2 cells and to explore the inhibitory mechanism of phycocyanin to tumor. METHODS: Highly purified phycocyanin was extracted from spirulina. The effects of phycocyanin at different concentrations on the growth of human laryngeal cancer HEP-2 cells were detected by MTT assay. In addition, the cell structures were observed under electron microscope. The cell apoptosis was analyzed by flow cytometry. The production of reactive oxygen species (ROS) was measured by flow cytometry. Enzymatic activities of caspase-3, -8 and -9 were measured by chemical colorimatry. The expression of Bax, Bcl-2, Fas, P53, caspase-3 and caspase-9 at mRNA and protein levels was determined by RT-PCR and Western blot. RESULTS: MTT test confirmed that phycocyanin inhibited the cell activity of HEP-2 cells with time and dose dependent manners. The result of electron microscope observation and flow cytometry indicated that phycocyanin induced the apoptosis of HEP-2 cells. The intracellular content of ROS was increased. The activities of caspase-3, -8 and -9 were increased. RT-PCR showed that the mRNA expression of Bax, Fas, P53, caspase-3, caspase-9 was increased and Bcl-2 was decreased. The results of Western blot were consistent with the results of RT-PCR. CONCLUSION: Phycocyanin might induce apoptosis of HEP-2 cells by down-regulating Bcl-2, up-regulating Bax, Fas and P53, and the transduction of apoptotic signals in the human laryngeal cancer cells. |
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| Keywords: | Phycocyanin Laryngeal cancer HEP-2 cells Apoptosis |
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