Expression,Purification and Activity Evaluation of Mb1230 Gene of Mycobacterium bovis |
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| Authors: | LI Ming JIA Hong XIN Ting GUO Xiao-yu YUAN Wei-feng HOU Shao-hua HOU Qiang GAO Xin-tao WU Jing DONG Rui-kai YANG Hong-jun LIU Lai-xing ZHU Hong-fei |
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| Institution: | 1. Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China;2. Dairy Cattle Research Center, Shandong Academy of Agricultural Sciences, Jinan 250183, China |
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| Abstract: | To explore the value of Mb1230 protein of Mycobacterium bovis in the diagnosis of bovine tuberculosis,we obtained the Mb1230 gene by PCR and constructed recombinant plasmid pET-22b-Mb1230.Recombinant Mb1230 protein was obtained by IPTG induction and purified by affinity chromatography.The activity of the recombinant protein was evaluated by TST test,IGRA test and indirect ELISA.The size of the recombinant protein matched with the theoretical value proved by SDS-PAGE;Western blotting result showed that the recombinant protein could react with mouse anti-His antibody,and had specific band;The results of TST test,IGRA test and indirect ELISA test also showed the recombinant protein had antigenic activity.The results indicated the recombinant protein Mb1230 had good B cell and T cell activity,so,it had the potential application in the diagnosis of bovine tuberculosis. |
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| Keywords: | Mycobacterium bovis Mb1230 prokaryotic expression diagnosis of bovine tuberculosis |
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