Screening and Identification of the Cellulase-producing Fungus |
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| Authors: | SHI Tong-rui LIU Yu WANG Yan WANG Shuang LI Dan CHEN Xi QIN Ping-wei |
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| Institution: | Heilongjiang Institute of Veterinary Medicine Science, Qiqihaer 161006, China |
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| Abstract: | In order to isolate the strains which could degrade fiber from the straw,the straw samples were inoculated on the potato agar medium and cultured at room temperature.The isolating strains were inoculated on the cellulose-congo red agar medium,the strains were screened that could produce bigger cellulose-decomposing zone.It was identified by morphological characteristics and molecular biological methods,and characterization of cellulase was analyzed preliminarily.The results showed that the fungus was Aspergillus niger,it could grow at room temperature,and the H/C was 7.64.The highest cellulase activity reached 42.18 U/mL when cultivated at 20 ℃ for 5 days.The optimum pH was 7.0 and the optimum reaction temperature was 20 ℃,the relative cellulase activity still retained above 90% at 20 to 40 ℃ or pH 6.0 to 8.0 for 1 h.The Aspergillus niger was a cold-adapted cellulaseproducing strain,it had strongger producing cellulase ability,and was tremendous potential valuable for microbial development. |
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| Keywords: | straw degradation Aspergillus niger cellulose |
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