Effects of miRNA-25 on proliferation of human esophageal squamous-cell carcinoma cell line TE1 |
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| Authors: | ZHANG Wei ZHOU Yong-hui PANG Yi-qiang |
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| Institution: | 1. Department of Pathophysiology, Baotou Medical College, Inner Mongolia University of Technology, Baotou 014010, China;
2. Department of Pathology, First Affiliated Hospital, Baotou Medical College, Inner Mongolia University of Technology, Baotou 014010, China;
3. Ward Ⅱ of General Surgery Department, First Affiliated Hospital, Baotou Medical College, Inner Mongolia University of Technology, Baotou 014010, China;
4. Department of Neurosurgery, Fourth Hospital of Baotou, Baotou 014030, China |
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| Abstract: | AIM: To investigate the effects and mechanisms of microRNA-25(miRNA-25) on the proliferation of human esophageal squamous-cell carcinoma cell line TE1. METHODS: The abundance of miRNA-25 in different tissues was measured by RT-PCR. After silencing or over-expression of miRNA-25 with mimics or inhibitor in TE1 cells, the cell proliferation, cell cycle distribution and the expression of cyclin E1 and cyclin-dependent kinase 2(CDK2) at mRNA and protein levels were measured by CCK-8 assay, BrdU detection, flow cytometry, RT-PCR and Western blot, respectively. RESULTS: miRNA-25 was prominent in esophageal mucosal tissue and highly expressed in TE1 cells (P<0.05). Over-expression of miRNA-25 increased TE1 cell proliferation, promoted the cell cycle progression and enhanced the entrance of the cells into S phase (P<0.05). Inverse results were obtained after down-regulation of miRNA-25(P<0.05). Furthermore, the expression of cyclin E1 and CDK2 at mRNA and protein levels was significantly increased after over-expression of miRNA-25, but decreased after down-regulation of miRNA-25(P<0.05). CONCLUSION: miRNA-25 enhances cell cycle transition by increasing the expression of cyclin E1 and CDK2, thus accelerating TE1 cell proliferation. This study provides a novel mechanism by which miRNA-25 increases the proliferation of human esophageal squamous-cell carcinoma cell line TE1, suggesting that down-regulation of miRNA-25 may be a potential new therapeutic strategy for treating esophageal squamous-cell carcinoma. |
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| Keywords: | MicroRNA-25 Human esophageal squamous-cell carcinoma cell line TE1 Cell proliferation Cyclin E1 Cyclin-dependent kinase 2 |
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