Advance of Cultural Methods for in vitro Spermatogenesis |
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Authors: | JI Meng LI Xiang-chen LI Lu GUAN Wei-jun MA Yue-hui |
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Institution: | 1. Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China;2. College of Animal Science and Technology, Agricultural University of Hebei, Baoding 071000, China |
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Abstract: | The generation of functional sperm in vitro has been a goal for almost a century.Fortunately,researchers reported the successful in vitro production of functional sperm,thereby potentially opening up a new era of reproductive biology.In vitro cultural methods for spermatogenesis include tissue cultural method and cells cultural method.Take together transplanting isolated cells into testicular tissue for tissue culture.Here,we summarize the history of research directed toward reproducing steps of spermatogenesis in vitro,suggest ways that can be applied toward clinical applications and address fundamental questions about the underlying mechanism of spermatogenesis. |
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Keywords: | in vitro spermatogenesis organ culture germ cells stem cells |
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