| 利用刃天青试剂快速检验普通白菜种子活力的研究 |
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| 引用本文： | 刘春香,李媛媛,王 萌,胡 畔.利用刃天青试剂快速检验普通白菜种子活力的研究[J].中国蔬菜,2015,1(7):49. |
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| 作者姓名： | 刘春香 李媛媛 王 萌 胡 畔 |
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| 作者单位： | （山东省高校生物化学与分子生物学重点实验室，潍坊学院，山东潍坊 261061） |
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| 基金项目： | 山东省自然科学基金项目（ZR2014JL017），潍坊学院青年基金项目（2013Z10） |
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| 摘 要： | 为了探索简单、快速、非破坏性检测普通白菜种子活力的方法,以苏州青、上海青种子为试材,44℃条件下人工老化处理0、2、4、6 d,测定种子活力指标及刃天青浸泡液颜色变化、吸光度,并进行相关性分析。结果表明:2个普通白菜品种的种子活力变化趋势相同,均随着种子老化程度的增加发芽率、发芽指数、活力指数明显下降;随着种子老化程度的加剧,刃天青浸泡液颜色从蓝色到粉色再到无色,吸光度亦明显下降;老化种子的发芽率与刃天青浸泡液吸光度、蓝色孔比例呈极显著正相关,相关系数均在0.96以上;老化种子的活力指数、发芽指数与刃天青浸泡液吸光度呈显著或极显著正相关。
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| 关 键 词： | 普通白菜 刃天青试剂 种子活力 吸光度 |
Studies on Quickly Testing Pakchoi Seed Vigor by Resazurin Reagent |
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| LIU Chun-Xiang,Li-Yuan-Yuan,Wang- Meng,Hu- Pan.Studies on Quickly Testing Pakchoi Seed Vigor by Resazurin Reagent[J].China Vegetables,2015,1(7):49. |
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| Authors: | LIU Chun-Xiang Li-Yuan-Yuan Wang- Meng Hu- Pan |
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| Institution: | （Key Laboratory of Biochemistry and Molecular Biology，Universities in Shandong Province，Weifang college，Weifang;
261061，Shandong，China） |
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| Abstract: | In order to explore simple，rapid and nondestructive method for testing pakchoi〔Brassica campestris L. ssp. chinensis（L.）Makino var.communisTsen et Lee〕seed vigor，‘Suzhouqing’and‘Shangha iqing’were used as experimental materials under 44 ℃ condition they underwent aging treatment for 0，2，4，6 days to test their seed vigor index and color change of resazurin reagent，absorbancy，and then correlation analysis was conducted．The result showed that the variation trend of these two pakchoi varieties were the same，their germination rate，germination index and vigor index were all dramatically declined along with the increase of aging degree．The color of resazurin reagent was changed from blue to pink then to colorless．The absorbance declined sharply．The seed germination rate，the absorbancy of resazurin reagent，blue hole rate were significantly positive correlated．The correlation coefficients were over 0.96．The seed vigor index，germination index and absorbancy of resazurin reagent were significantly or highly significantly positive correlated． |
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| Keywords: | Pakchoi Resazurin reagent Seed vigor Absorbance |
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