| 华中地区猪繁殖与呼吸综合征病毒临床流行毒株Nsp2和GP5氨基酸的遗传变异分析 |
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| 引用本文： | 李彬,王豪男,彭忠,胡睿铭,吴斌.华中地区猪繁殖与呼吸综合征病毒临床流行毒株Nsp2和GP5氨基酸的遗传变异分析[J].中国畜牧兽医,2015,42(11):3026-3036. |
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| 作者姓名： | 李彬 王豪男 彭忠 胡睿铭 吴斌 |
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| 作者单位： | 1. 华中农业大学农业微生物学国家重点实验室, 武汉 430070;2. 生猪健康养殖协同创新中心, 武汉 430070 |
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| 基金项目： | 湖北省科技支撑计划优质家畜新品种(系)培育及高效安全生产关键技术(2013BBB14);国家科技支撑计划项目优良种猪高效利用关键工程化技术集成与示范(2014BAD20B00) |
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| 摘 要： | 为了解当前猪繁殖与呼吸综合征病毒(porcine reproductive and respiratory syndrome virus,PRRSV)流行毒株的遗传变异情况,本研究对2013-2014年间来源于中国华中地区湖北、河南和湖南3省20个猪场65份疑似PRRSV感染的临床样品开展病毒分离工作,通过RT-PCR及间接免疫荧光试验进行鉴定,结果显示成功分离到4株PRRSV。通过测序获得它们的部分Nsp2氨基酸序列,经比对分析,确定分离毒株皆为变异毒株,并且与JXA1、TJ等高致病性毒株有相同的遗传特点,在Nsp2区域有30个氨基酸的不连续缺失。本研究将从临床样品中测序获得的23株PRRSV的GP5氨基酸序列与GenBank公布的有代表性的毒株序列进行遗传进化分析,结果显示所有毒株均为美洲型毒株,其中21株与高致病性变异毒株(JXA1、TJ和HUN4)同源性较高,同时在主要中和位点和N-糖基化位点存在部分氨基酸突变,另外两株与疫苗毒株RespPRRS MLV同源性较高。本试验结果表明,当前高致病性PRRSV依然是优势毒株,其GP5氨基酸突变频繁,不同地区流行毒株存在一定的遗传差异。
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| 关 键 词： | 猪繁殖与呼吸综合征 分离鉴定 序列分析 |
| 收稿时间： | 2015-06-26 |
Genetic Variation Analysis of Nsp2 and GP5 Amino Acids of Porcine Reproductive and Respiratory Syndrome Virus in Clinical in Central China |
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| LI Bin,WANG Hao-nan,PENG Zhong,HU Rui-ming,WU Bin.Genetic Variation Analysis of Nsp2 and GP5 Amino Acids of Porcine Reproductive and Respiratory Syndrome Virus in Clinical in Central China[J].China Animal Husbandry & Veterinary Medicine,2015,42(11):3026-3036. |
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| Authors: | LI Bin WANG Hao-nan PENG Zhong HU Rui-ming WU Bin |
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| Institution: | 1. State Key Laboratory of Agricultural Microbiology of Huazhong Agricultural University, Wuhan 430070, China;2. Cooperative Innovation Centerfor Sustainable Pig Production, Wuhan 430070, China |
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| Abstract: | In order to disclose the genetic variation of PRRSV,65 materials from some areas were identified during the period from 2013 to 2014.Viruses were isolated from the suspected PRRS samples.Four isolates were isolated and identified by RT-PCR and IFA.Partial Nsp2 amino acid sequences of the isolates were obtained by sequencing,and comparison analysis showed that all of the isolates were variant strains.Then we found a discontinuous deletion of 30 amino acids in Nsp2.It shared the same feature with JXA1 and TJ.Besides,we sequenced and analyzed the GP5 amino acids of 23 currently epidemic strains with some typical strains published online earlier,the results showed that all strains were American strain,21 of which had high homology with highly pathogenic variant strains (JXA1,TJ and HUN4).Moreover,further analysis of the GP5 protein suggested that these strains exhibited variations in the primary neutralizing epitope and N-linked glycosylation sites.The other two strains had the same genetic features with RespPRRSV MLV.The results showed that the highly pathogenic PRRSV was still the dominant strain and GP5 amino acid mutations were frequently,there were some genetic differences between PRRSV in different regions. |
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| Keywords: | porcine reproductive and respiratory syndrome isolation and identification sequence analysis |
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